TY - JOUR
T1 - Characterization of heat-induced interaction of neutral liposome with lipid membrane of Streptomyces griseus cell
AU - Ngo, Kien Xuan
AU - Umakoshi, Hiroshi
AU - Shimanouchi, Toshinori
AU - Kuboi, Ryoichi
N1 - Funding Information:
The fundamental concept of this study was supported by the Research Group of “Membrane Stress Biotechnology”. It was partly supported by a Grant-in-Aid for Scientific Research (No. 15206089, 16686046, 16760635, 17656268, 19656203, 19656220, and 20360350) from the Ministry of Education, Science, Sports, and Culture of Japan, a grant from the 21st Century COE program “Creation of Integrated EcoChemistry” and the Global COE program “Bio-Environmental Chemistry” of the Japan Society for the Promotion of Science (JSPS). The authors are grateful to the Research Center for Solar Energy Chemistry of Osaka University and the Gas hydrate Analyzing System of Osaka University. K.X. Ngo acknowledges the financial support from Japanese Society for Promotion of Science.
PY - 2009/10/15
Y1 - 2009/10/15
N2 - The interaction between the neutral 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) liposomes and cell membrane of Streptomyces griseus induced by the heat treatment at specific temperature was investigated, focusing on the internalization of the neutral POPC liposomes with S. griseus cells. In an attempt to clarify the modes of liposome internalization, various kinds of inhibitors of endocytotic pathways were used to treat S. griseus cells. The efficiency of the heat treatment on liposome-cell membrane interactions was finally characterized based on the hydrophobic, electrostatic interactions and hydration effect. In fact, the internalization of the neutral liposomes into these cells was found to show higher rate and greater amount at higher temperatures. The kinetic study showed that the maximum amount of the internalized liposomes was, respectively, 469 × 105 and 643 × 105 liposomes/cell at 37 and 41 °C. The internalization of the neutral liposomes induced by the heat treatment was characterized, implying that the endocytosis occurred. The interactions involving the internalization, adsorption, and fusion of these liposomes with S. griseus cells were mainly contributed by the hydrophobic interaction and the unstable hydrogen bonds caused by the loss of water of surface hydration of cell membrane rather than the electrostatic interaction under the specific heat condition.
AB - The interaction between the neutral 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) liposomes and cell membrane of Streptomyces griseus induced by the heat treatment at specific temperature was investigated, focusing on the internalization of the neutral POPC liposomes with S. griseus cells. In an attempt to clarify the modes of liposome internalization, various kinds of inhibitors of endocytotic pathways were used to treat S. griseus cells. The efficiency of the heat treatment on liposome-cell membrane interactions was finally characterized based on the hydrophobic, electrostatic interactions and hydration effect. In fact, the internalization of the neutral liposomes into these cells was found to show higher rate and greater amount at higher temperatures. The kinetic study showed that the maximum amount of the internalized liposomes was, respectively, 469 × 105 and 643 × 105 liposomes/cell at 37 and 41 °C. The internalization of the neutral liposomes induced by the heat treatment was characterized, implying that the endocytosis occurred. The interactions involving the internalization, adsorption, and fusion of these liposomes with S. griseus cells were mainly contributed by the hydrophobic interaction and the unstable hydrogen bonds caused by the loss of water of surface hydration of cell membrane rather than the electrostatic interaction under the specific heat condition.
KW - Heat stress
KW - Internalization
KW - Membrane interaction
KW - Neutral liposomes
KW - Streptomyces griseus
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U2 - 10.1016/j.colsurfb.2009.06.015
DO - 10.1016/j.colsurfb.2009.06.015
M3 - Article
C2 - 19592227
AN - SCOPUS:68549128504
SN - 0927-7765
VL - 73
SP - 399
EP - 407
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
IS - 2
ER -