TY - JOUR
T1 - Characterization of in vivo effects of platelet-rich plasma and biodegradable gelatin hydrogel microspheres on degenerated intervertebral discs
AU - Sawamura, Kazuhide
AU - Ikeda, Takumi
AU - Nagae, Masateru
AU - Okamoto, Shin Ichi
AU - Mikami, Yasuo
AU - Hase, Hitoshi
AU - Ikoma, Kazuya
AU - Yamada, Tetsuya
AU - Sakamoto, Hirotaka
AU - Matsuda, Ken Ichi
AU - Tabata, Yasuhiko
AU - Kawata, Mitsuhiro
AU - Kubo, Toshikazu
PY - 2009/12/1
Y1 - 2009/12/1
N2 - We have previously shown that administration of platelet-rich plasma-impregnated gelatin hydrogel microspheres (PRP-GHMs) into a degenerated intervertebral disc (IVD) markedly suppresses progression of IVD degeneration. In the current study, we characterized the in vivo effects of PRP-GHM treatment in a degenerated IVD model in rabbit. On magnetic resonance images, the IVD height was significantly greater after treatment with PRP-GHMs compared with phosphate-buffered saline-impregnated GHMs, PRP without GHMs, and needle puncture only. Water content was also preserved in PRP-GHM-treated IVDs. Consistent with this observation, the mRNA expression of proteoglycan core protein and type II collagen was significantly higher after PRP-GHM treatment compared with other treatment groups. No proliferating cells were found in the nucleus pulposus and inner annulus fibrosus in any groups, but the number of apoptotic cells in the nucleus pulposus after PRP-GHM treatment was significantly lower than that after other treatments. These results provide an improved understanding of the therapeutic effects of PRP-GHM treatment of degenerated IVDs.
AB - We have previously shown that administration of platelet-rich plasma-impregnated gelatin hydrogel microspheres (PRP-GHMs) into a degenerated intervertebral disc (IVD) markedly suppresses progression of IVD degeneration. In the current study, we characterized the in vivo effects of PRP-GHM treatment in a degenerated IVD model in rabbit. On magnetic resonance images, the IVD height was significantly greater after treatment with PRP-GHMs compared with phosphate-buffered saline-impregnated GHMs, PRP without GHMs, and needle puncture only. Water content was also preserved in PRP-GHM-treated IVDs. Consistent with this observation, the mRNA expression of proteoglycan core protein and type II collagen was significantly higher after PRP-GHM treatment compared with other treatment groups. No proliferating cells were found in the nucleus pulposus and inner annulus fibrosus in any groups, but the number of apoptotic cells in the nucleus pulposus after PRP-GHM treatment was significantly lower than that after other treatments. These results provide an improved understanding of the therapeutic effects of PRP-GHM treatment of degenerated IVDs.
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U2 - 10.1089/ten.tea.2008.0697
DO - 10.1089/ten.tea.2008.0697
M3 - Article
C2 - 19514846
AN - SCOPUS:72049121634
SN - 1937-3341
VL - 15
SP - 3719
EP - 3727
JO - Tissue Engineering - Part A.
JF - Tissue Engineering - Part A.
IS - 12
ER -