Characterization of Mesenchymal Stem Cell-Like Cells Derived from Human iPSCs via Neural Crest Development and Their Application for Osteochondral Repair

Ryota Chijimatsu, Makoto Ikeya, Yukihiko Yasui, Yasutoshi Ikeda, Kosuke Ebina, Yu Moriguchi, Kazunori Shimomura, David A. Hart, Yoshikawa Hideki, Nakamura Norimasa

Research output: Contribution to journalArticlepeer-review

56 Citations (Scopus)

Abstract

Mesenchymal stem cells (MSCs) derived from induced pluripotent stem cells (iPSCs) are a promising cell source for the repair of skeletal disorders. Recently, neural crest cells (NCCs) were reported to be effective for inducing mesenchymal progenitors, which have potential to differentiate into osteochondral lineages. Our aim was to investigate the feasibility of MSC-like cells originated from iPSCs via NCCs for osteochondral repair. Initially, MSC-like cells derived from iPSC-NCCs (iNCCs) were generated and characterized in vitro. These iNCC-derived MSC-like cells (iNCMSCs) exhibited a homogenous population and potential for osteochondral differentiation. No upregulation of pluripotent markers was detected during culture. Second, we implanted iNCMSC-derived tissue-engineered constructs into rat osteochondral defects without any preinduction for specific differentiation lineages. The implanted cells remained alive at the implanted site, whereas they failed to repair the defects, with only scarce development of osteochondral tissue in vivo. With regard to tumorigenesis, the implanted cells gradually disappeared and no malignant cells were detected throughout the 2-month follow-up. While this study did not show that iNCMSCs have efficacy for repair of osteochondral defects when implanted under undifferentiated conditions, iNCMSCs exhibited good chondrogenic potential in vitro under appropriate conditions. With further optimization, iNCMSCs may be a new source for tissue engineering of cartilage.

Original languageEnglish
Article number1960965
JournalStem Cells International
Volume2017
DOIs
Publication statusPublished - 2017
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Characterization of Mesenchymal Stem Cell-Like Cells Derived from Human iPSCs via Neural Crest Development and Their Application for Osteochondral Repair'. Together they form a unique fingerprint.

Cite this