Characterization of Pseudomonas aeruginosa phage KPP21 belonging to family Podoviridae genus N4-like viruses isolated in Japan

Ryu Shigehisa; Jumpei Uchiyama; Shin ichiro Kato; Iyo Takemura-Uchiyama; Kotoe Yamaguchi; Reina Miyata; Takako Ujihara; Yoshihiko Sakaguchi; Noriaki Okamoto; Hidekatsu Shimakura; Masanori Daibata; Masahiro Sakaguchi; Shigenobu Matsuzaki

doi:10.1111/1348-0421.12347

Characterization of Pseudomonas aeruginosa phage KPP21 belonging to family Podoviridae genus N4-like viruses isolated in Japan

Ryu Shigehisa, Jumpei Uchiyama, Shin ichiro Kato, Iyo Takemura-Uchiyama, Kotoe Yamaguchi, Reina Miyata, Takako Ujihara, Yoshihiko Sakaguchi, Noriaki Okamoto, Hidekatsu Shimakura, Masanori Daibata, Masahiro Sakaguchi, Shigenobu Matsuzaki

Research output: Contribution to journal › Comment/debate › peer-review

10 Citations (Scopus)

Abstract

Bacteriophages (phages) belonging to the family Podoviridae genus N4-like viruses have been used as therapeutic agent in phage therapy against Pseudomonas aeruginosa infections. P. aeruginosa phage KPP21 was isolated in Japan, and phylogenetically investigated the phages belonging to this viral genus. Morphological and genetic analyses confirmed that phage KPP21 belongs to the family Podoviridae genus N4-like viruses. Moreover, phylogenetic analyses based on putative DNA polymerase and major virion protein showed that P. aeruginosa phages belonging to the genus N4-like viruses are separated into two lineages and that phage KPP21 is in the same clade as phage LUZ7.

Original language	English
Pages (from-to)	64-67
Number of pages	4
Journal	MICROBIOLOGY and IMMUNOLOGY
Volume	60
Issue number	1
DOIs	https://doi.org/10.1111/1348-0421.12347
Publication status	Published - Jan 1 2016
Externally published	Yes

Keywords

Bacteriophage
Phage therapy
Phylogeny
Pseudomonas aeruginosa

ASJC Scopus subject areas

Microbiology
Immunology
Virology

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Shigehisa, R., Uchiyama, J., Kato, S. I., Takemura-Uchiyama, I., Yamaguchi, K., Miyata, R., Ujihara, T., Sakaguchi, Y., Okamoto, N., Shimakura, H., Daibata, M., Sakaguchi, M., & Matsuzaki, S. (2016). Characterization of Pseudomonas aeruginosa phage KPP21 belonging to family Podoviridae genus N4-like viruses isolated in Japan. MICROBIOLOGY and IMMUNOLOGY, 60(1), 64-67. https://doi.org/10.1111/1348-0421.12347

Shigehisa, R, Uchiyama, J, Kato, SI, Takemura-Uchiyama, I, Yamaguchi, K, Miyata, R, Ujihara, T, Sakaguchi, Y, Okamoto, N, Shimakura, H, Daibata, M, Sakaguchi, M & Matsuzaki, S 2016, 'Characterization of Pseudomonas aeruginosa phage KPP21 belonging to family Podoviridae genus N4-like viruses isolated in Japan', MICROBIOLOGY and IMMUNOLOGY, vol. 60, no. 1, pp. 64-67. https://doi.org/10.1111/1348-0421.12347

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abstract = "Bacteriophages (phages) belonging to the family Podoviridae genus N4-like viruses have been used as therapeutic agent in phage therapy against Pseudomonas aeruginosa infections. P. aeruginosa phage KPP21 was isolated in Japan, and phylogenetically investigated the phages belonging to this viral genus. Morphological and genetic analyses confirmed that phage KPP21 belongs to the family Podoviridae genus N4-like viruses. Moreover, phylogenetic analyses based on putative DNA polymerase and major virion protein showed that P. aeruginosa phages belonging to the genus N4-like viruses are separated into two lineages and that phage KPP21 is in the same clade as phage LUZ7.",

keywords = "Bacteriophage, Phage therapy, Phylogeny, Pseudomonas aeruginosa",

author = "Ryu Shigehisa and Jumpei Uchiyama and Kato, {Shin ichiro} and Iyo Takemura-Uchiyama and Kotoe Yamaguchi and Reina Miyata and Takako Ujihara and Yoshihiko Sakaguchi and Noriaki Okamoto and Hidekatsu Shimakura and Masanori Daibata and Masahiro Sakaguchi and Shigenobu Matsuzaki",

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doi = "10.1111/1348-0421.12347",

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AU - Shigehisa, Ryu

AU - Uchiyama, Jumpei

AU - Kato, Shin ichiro

AU - Takemura-Uchiyama, Iyo

AU - Yamaguchi, Kotoe

AU - Miyata, Reina

AU - Ujihara, Takako

AU - Sakaguchi, Yoshihiko

AU - Okamoto, Noriaki

AU - Shimakura, Hidekatsu

AU - Daibata, Masanori

AU - Sakaguchi, Masahiro

AU - Matsuzaki, Shigenobu

PY - 2016/1/1

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AB - Bacteriophages (phages) belonging to the family Podoviridae genus N4-like viruses have been used as therapeutic agent in phage therapy against Pseudomonas aeruginosa infections. P. aeruginosa phage KPP21 was isolated in Japan, and phylogenetically investigated the phages belonging to this viral genus. Morphological and genetic analyses confirmed that phage KPP21 belongs to the family Podoviridae genus N4-like viruses. Moreover, phylogenetic analyses based on putative DNA polymerase and major virion protein showed that P. aeruginosa phages belonging to the genus N4-like viruses are separated into two lineages and that phage KPP21 is in the same clade as phage LUZ7.

KW - Bacteriophage

KW - Phage therapy

KW - Phylogeny

KW - Pseudomonas aeruginosa

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Characterization of Pseudomonas aeruginosa phage KPP21 belonging to family Podoviridae genus N4-like viruses isolated in Japan

Abstract

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