Characterization of the quinones in purple sulfur bacterium Thermochromatium tepidum

Yuuka Kimura; Tomoaki Kawakami; Long Jiang Yu; Miku Yoshimura; Masayuki Kobayashi; Zheng Yu Wang-Otomo

doi:10.1016/j.febslet.2015.05.043

Characterization of the quinones in purple sulfur bacterium Thermochromatium tepidum

Yuuka Kimura, Tomoaki Kawakami, Long Jiang Yu, Miku Yoshimura, Masayuki Kobayashi, Zheng Yu Wang-Otomo

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Abstract Quinone distributions in the thermophilic purple sulfur bacterium Thermochromatium tepidum have been investigated at different levels of the photosynthetic apparatus. Here we show that, on average, the intracytoplasmic membrane contains 18 ubiquinones (UQ) and 4 menaquinones (MQ) per reaction center (RC). About one-third of the quinones are retained in the light-harvesting-reaction center core complex (LH1-RC) with a similar ratio of UQ to MQ. The numbers of quinones essentially remains unchanged during crystallization of the LH1-RC. There are 1-2 UQ and 1 MQ associated with the RC-only complex in the purified solution sample. Our results suggest that a large proportion of the quinones are confined to the core complex and at least five UQs remain invisible in the current LH1-RC crystal structure.

Original language	English
Article number	37199
Pages (from-to)	1761-1765
Number of pages	5
Journal	FEBS Letters
Volume	589
Issue number	15
DOIs	https://doi.org/10.1016/j.febslet.2015.05.043
Publication status	Published - Jul 3 2015

Keywords

Chromatophore
Electron transport
Light-harvesting
Photosynthesis
Reaction center

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2015.05.043

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title = "Characterization of the quinones in purple sulfur bacterium Thermochromatium tepidum",

abstract = "Abstract Quinone distributions in the thermophilic purple sulfur bacterium Thermochromatium tepidum have been investigated at different levels of the photosynthetic apparatus. Here we show that, on average, the intracytoplasmic membrane contains 18 ubiquinones (UQ) and 4 menaquinones (MQ) per reaction center (RC). About one-third of the quinones are retained in the light-harvesting-reaction center core complex (LH1-RC) with a similar ratio of UQ to MQ. The numbers of quinones essentially remains unchanged during crystallization of the LH1-RC. There are 1-2 UQ and 1 MQ associated with the RC-only complex in the purified solution sample. Our results suggest that a large proportion of the quinones are confined to the core complex and at least five UQs remain invisible in the current LH1-RC crystal structure.",

keywords = "Chromatophore, Electron transport, Light-harvesting, Photosynthesis, Reaction center",

author = "Yuuka Kimura and Tomoaki Kawakami and Yu, {Long Jiang} and Miku Yoshimura and Masayuki Kobayashi and Wang-Otomo, {Zheng Yu}",

note = "Funding Information: We thank Y. Takasu and S. Igarashi for experimental assistance. This work was supported in part by Grant for Basic Scientific Research Projects from The Sumitomo Foundation and The Kurata Memorial Hitachi Science and Technology Foundation . We thank Kao Corporation for kindly providing the LDAO. Publisher Copyright: {\textcopyright} 2015 Federation of European Biochemical Societies.",

year = "2015",

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doi = "10.1016/j.febslet.2015.05.043",

language = "English",

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T1 - Characterization of the quinones in purple sulfur bacterium Thermochromatium tepidum

AU - Kimura, Yuuka

AU - Kawakami, Tomoaki

AU - Yu, Long Jiang

AU - Yoshimura, Miku

AU - Kobayashi, Masayuki

AU - Wang-Otomo, Zheng Yu

N1 - Funding Information: We thank Y. Takasu and S. Igarashi for experimental assistance. This work was supported in part by Grant for Basic Scientific Research Projects from The Sumitomo Foundation and The Kurata Memorial Hitachi Science and Technology Foundation . We thank Kao Corporation for kindly providing the LDAO. Publisher Copyright: © 2015 Federation of European Biochemical Societies.

PY - 2015/7/3

Y1 - 2015/7/3

N2 - Abstract Quinone distributions in the thermophilic purple sulfur bacterium Thermochromatium tepidum have been investigated at different levels of the photosynthetic apparatus. Here we show that, on average, the intracytoplasmic membrane contains 18 ubiquinones (UQ) and 4 menaquinones (MQ) per reaction center (RC). About one-third of the quinones are retained in the light-harvesting-reaction center core complex (LH1-RC) with a similar ratio of UQ to MQ. The numbers of quinones essentially remains unchanged during crystallization of the LH1-RC. There are 1-2 UQ and 1 MQ associated with the RC-only complex in the purified solution sample. Our results suggest that a large proportion of the quinones are confined to the core complex and at least five UQs remain invisible in the current LH1-RC crystal structure.

AB - Abstract Quinone distributions in the thermophilic purple sulfur bacterium Thermochromatium tepidum have been investigated at different levels of the photosynthetic apparatus. Here we show that, on average, the intracytoplasmic membrane contains 18 ubiquinones (UQ) and 4 menaquinones (MQ) per reaction center (RC). About one-third of the quinones are retained in the light-harvesting-reaction center core complex (LH1-RC) with a similar ratio of UQ to MQ. The numbers of quinones essentially remains unchanged during crystallization of the LH1-RC. There are 1-2 UQ and 1 MQ associated with the RC-only complex in the purified solution sample. Our results suggest that a large proportion of the quinones are confined to the core complex and at least five UQs remain invisible in the current LH1-RC crystal structure.

KW - Chromatophore

KW - Electron transport

KW - Light-harvesting

KW - Photosynthesis

KW - Reaction center

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Characterization of the quinones in purple sulfur bacterium Thermochromatium tepidum

Abstract

Keywords

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