Characterization of the two centromeric proteins CENP-C and MIS12 in Nicotiana species

Kiyotaka Nagaki; Kazunari Kashihara; Minoru Murata

doi:10.1007/s10577-009-9064-8

Characterization of the two centromeric proteins CENP-C and MIS12 in Nicotiana species

Kiyotaka Nagaki, Kazunari Kashihara, Minoru Murata

Institute of Plant Science and Resources

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Centromeres play an important role in chromosome transmission in eukaryotes and comprise specific DNA and proteins that form complexes called kinetochores. In tobacco, although a centromere-specific histone H3 (NtCENH3) and centromeric DNA sequence (Nt2-7) have been identified, no other kinetochore components have been determined. In this study, we isolated and characterized cDNAs encoding two centromeric proteins CENP-C and MIS12 from Nicotiana tabaccum. Two CENP-C homologues, NtCENP-C-1 and -2, isolated from N. tabaccum were similar to CENP-C from N. sylvestris and N. tomentosiformis, respectively. Similarly, two Mis12 homologues, NtMIS12-1 and -2, in N. tabaccum were shown to originate from N. sylvestris and N. tomentosiformis, respectively. Both respective homologues for CENP-C and Mis12 were expressed at the same level. This indicates that in a tetraploid species, N. tabaccum, two ancestral genes encoding the centromeric proteins participate equally in the functioning of centromeres.

Original language	English
Pages (from-to)	719-726
Number of pages	8
Journal	Chromosome Research
Volume	17
Issue number	6
DOIs	https://doi.org/10.1007/s10577-009-9064-8
Publication status	Published - Aug 2009

Keywords

CENP-C
Centromere
MIS12
Nicotiana
Tobacco

ASJC Scopus subject areas

Genetics

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10.1007/s10577-009-9064-8

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title = "Characterization of the two centromeric proteins CENP-C and MIS12 in Nicotiana species",

abstract = "Centromeres play an important role in chromosome transmission in eukaryotes and comprise specific DNA and proteins that form complexes called kinetochores. In tobacco, although a centromere-specific histone H3 (NtCENH3) and centromeric DNA sequence (Nt2-7) have been identified, no other kinetochore components have been determined. In this study, we isolated and characterized cDNAs encoding two centromeric proteins CENP-C and MIS12 from Nicotiana tabaccum. Two CENP-C homologues, NtCENP-C-1 and -2, isolated from N. tabaccum were similar to CENP-C from N. sylvestris and N. tomentosiformis, respectively. Similarly, two Mis12 homologues, NtMIS12-1 and -2, in N. tabaccum were shown to originate from N. sylvestris and N. tomentosiformis, respectively. Both respective homologues for CENP-C and Mis12 were expressed at the same level. This indicates that in a tetraploid species, N. tabaccum, two ancestral genes encoding the centromeric proteins participate equally in the functioning of centromeres.",

keywords = "CENP-C, Centromere, MIS12, Nicotiana, Tobacco",

author = "Kiyotaka Nagaki and Kazunari Kashihara and Minoru Murata",

note = "Funding Information: Acknowledgements This work was supported by a grant from the Ministry of Education, Culture, Sports, Science and Technology in Japan (#18770007). Seeds of N. sylvestris and N. tomentosiformis were kindly provided by Japan Tobacco Inc.",

year = "2009",

month = aug,

doi = "10.1007/s10577-009-9064-8",

language = "English",

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pages = "719--726",

journal = "Chromosome Research",

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T1 - Characterization of the two centromeric proteins CENP-C and MIS12 in Nicotiana species

AU - Nagaki, Kiyotaka

AU - Kashihara, Kazunari

AU - Murata, Minoru

N1 - Funding Information: Acknowledgements This work was supported by a grant from the Ministry of Education, Culture, Sports, Science and Technology in Japan (#18770007). Seeds of N. sylvestris and N. tomentosiformis were kindly provided by Japan Tobacco Inc.

PY - 2009/8

Y1 - 2009/8

N2 - Centromeres play an important role in chromosome transmission in eukaryotes and comprise specific DNA and proteins that form complexes called kinetochores. In tobacco, although a centromere-specific histone H3 (NtCENH3) and centromeric DNA sequence (Nt2-7) have been identified, no other kinetochore components have been determined. In this study, we isolated and characterized cDNAs encoding two centromeric proteins CENP-C and MIS12 from Nicotiana tabaccum. Two CENP-C homologues, NtCENP-C-1 and -2, isolated from N. tabaccum were similar to CENP-C from N. sylvestris and N. tomentosiformis, respectively. Similarly, two Mis12 homologues, NtMIS12-1 and -2, in N. tabaccum were shown to originate from N. sylvestris and N. tomentosiformis, respectively. Both respective homologues for CENP-C and Mis12 were expressed at the same level. This indicates that in a tetraploid species, N. tabaccum, two ancestral genes encoding the centromeric proteins participate equally in the functioning of centromeres.

AB - Centromeres play an important role in chromosome transmission in eukaryotes and comprise specific DNA and proteins that form complexes called kinetochores. In tobacco, although a centromere-specific histone H3 (NtCENH3) and centromeric DNA sequence (Nt2-7) have been identified, no other kinetochore components have been determined. In this study, we isolated and characterized cDNAs encoding two centromeric proteins CENP-C and MIS12 from Nicotiana tabaccum. Two CENP-C homologues, NtCENP-C-1 and -2, isolated from N. tabaccum were similar to CENP-C from N. sylvestris and N. tomentosiformis, respectively. Similarly, two Mis12 homologues, NtMIS12-1 and -2, in N. tabaccum were shown to originate from N. sylvestris and N. tomentosiformis, respectively. Both respective homologues for CENP-C and Mis12 were expressed at the same level. This indicates that in a tetraploid species, N. tabaccum, two ancestral genes encoding the centromeric proteins participate equally in the functioning of centromeres.

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KW - MIS12

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KW - Tobacco

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Characterization of the two centromeric proteins CENP-C and MIS12 in Nicotiana species

Abstract

Keywords

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