Chemical modification of cytosine residues of mouse 5 S ribosomal RNA with hydrogen sulfide (Nucleosides and nucleotides 43)

Kazunobu Miura; Shigeki Tsuda; Tatsuyoshi Iwano; Tohru Ueda; Fumio Harada; Nobuyuki Kato

doi:10.1016/0167-4781(83)90028-3

Chemical modification of cytosine residues of mouse 5 S ribosomal RNA with hydrogen sulfide (Nucleosides and nucleotides 43)

Kazunobu Miura, Shigeki Tsuda, Tatsuyoshi Iwano, Tohru Ueda, Fumio Harada, Nobuyuki Kato

Graduate School of Medicine Dentistry and Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Cytosine residues of nucleic acids were converted to 4-thiouracil residues with hydrogen sulfide in pyridine and water to examine the secondary and tertiary structures of mouse 5 S rRNA. The cytosine residues at positions 10, 24, 34 (or 36), 39, 44 (or 46) and 63 were converted preferentially when the treatment was carried out at 28°C. This result supports the model of the secondary structure of 5 S rRNA of Nishikawa, K. and Takemura, S. ((1974) FEBS Lett. 40, 106-109) consisting of five helices and five loops. As the temperature was increased to 35°C, additional cytosine residues in positions 26, 52 and 78 were modified to moderate extents.

Original language	English
Pages (from-to)	181-189
Number of pages	9
Journal	BBA - Gene Structure and Expression
Volume	739
Issue number	2
DOIs	https://doi.org/10.1016/0167-4781(83)90028-3
Publication status	Published - Mar 10 1983

Keywords

(Mouse kidney)
Chemical modification
Cytosine
Hydrogen sulfide
Thiouracil
rRNA

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/0167-4781(83)90028-3

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title = "Chemical modification of cytosine residues of mouse 5 S ribosomal RNA with hydrogen sulfide (Nucleosides and nucleotides 43)",

abstract = "Cytosine residues of nucleic acids were converted to 4-thiouracil residues with hydrogen sulfide in pyridine and water to examine the secondary and tertiary structures of mouse 5 S rRNA. The cytosine residues at positions 10, 24, 34 (or 36), 39, 44 (or 46) and 63 were converted preferentially when the treatment was carried out at 28°C. This result supports the model of the secondary structure of 5 S rRNA of Nishikawa, K. and Takemura, S. ((1974) FEBS Lett. 40, 106-109) consisting of five helices and five loops. As the temperature was increased to 35°C, additional cytosine residues in positions 26, 52 and 78 were modified to moderate extents.",

keywords = "(Mouse kidney), Chemical modification, Cytosine, Hydrogen sulfide, Thiouracil, rRNA",

author = "Kazunobu Miura and Shigeki Tsuda and Tatsuyoshi Iwano and Tohru Ueda and Fumio Harada and Nobuyuki Kato",

note = "Funding Information: This work was supported by a Grant-in-aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.",

year = "1983",

month = mar,

day = "10",

doi = "10.1016/0167-4781(83)90028-3",

language = "English",

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TY - JOUR

T1 - Chemical modification of cytosine residues of mouse 5 S ribosomal RNA with hydrogen sulfide (Nucleosides and nucleotides 43)

AU - Miura, Kazunobu

AU - Tsuda, Shigeki

AU - Iwano, Tatsuyoshi

AU - Ueda, Tohru

AU - Harada, Fumio

AU - Kato, Nobuyuki

N1 - Funding Information: This work was supported by a Grant-in-aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.

PY - 1983/3/10

Y1 - 1983/3/10

N2 - Cytosine residues of nucleic acids were converted to 4-thiouracil residues with hydrogen sulfide in pyridine and water to examine the secondary and tertiary structures of mouse 5 S rRNA. The cytosine residues at positions 10, 24, 34 (or 36), 39, 44 (or 46) and 63 were converted preferentially when the treatment was carried out at 28°C. This result supports the model of the secondary structure of 5 S rRNA of Nishikawa, K. and Takemura, S. ((1974) FEBS Lett. 40, 106-109) consisting of five helices and five loops. As the temperature was increased to 35°C, additional cytosine residues in positions 26, 52 and 78 were modified to moderate extents.

AB - Cytosine residues of nucleic acids were converted to 4-thiouracil residues with hydrogen sulfide in pyridine and water to examine the secondary and tertiary structures of mouse 5 S rRNA. The cytosine residues at positions 10, 24, 34 (or 36), 39, 44 (or 46) and 63 were converted preferentially when the treatment was carried out at 28°C. This result supports the model of the secondary structure of 5 S rRNA of Nishikawa, K. and Takemura, S. ((1974) FEBS Lett. 40, 106-109) consisting of five helices and five loops. As the temperature was increased to 35°C, additional cytosine residues in positions 26, 52 and 78 were modified to moderate extents.

KW - (Mouse kidney)

KW - Chemical modification

KW - Cytosine

KW - Hydrogen sulfide

KW - Thiouracil

KW - rRNA

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U2 - 10.1016/0167-4781(83)90028-3

DO - 10.1016/0167-4781(83)90028-3

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AN - SCOPUS:0020597012

SN - 0167-4781

VL - 739

SP - 181

EP - 189

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

IS - 2

ER -

Chemical modification of cytosine residues of mouse 5 S ribosomal RNA with hydrogen sulfide (Nucleosides and nucleotides 43)

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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