Chemoattractant efficacy: Oxidation of stimulus by responding cells

Teizo Yoshimura; Antal Rot; Edward J. Leonard

doi:10.1016/0006-291X(86)90247-0

Chemoattractant efficacy: Oxidation of stimulus by responding cells

Teizo Yoshimura, Antal Rot, Edward J. Leonard

Research output: Contribution to journal › Article › peer-review

Abstract

Although all human neutrophils have receptors for formyl-methionyl-leucyl-phenylalanine, only 20-30% migrate in vitro at optimal attractant concentrations. Since this attractant can be oxidized by myeloperoxidase from stimulated neutrophils, we determined if its efficacy was increased by protection from oxidation. Efficacy was increased by reducing agents and by molecules with oxidizable sulfur, 10^-5M methionine or 1.5×10^-6M albumin. The antioxidant effect was on the attractant, not the cells: albumin in the cell compartment did not increase responses. Furthermore, antioxidants had no effect on efficacy of fNle-Leu-Phe-Nle-Tyr-Lys, an attractant that also stimulated superoxide release but had no oxidizable sulfur.

Original language	English
Pages (from-to)	66-71
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	138
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(86)90247-0
Publication status	Published - Jul 16 1986
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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abstract = "Although all human neutrophils have receptors for formyl-methionyl-leucyl-phenylalanine, only 20-30% migrate in vitro at optimal attractant concentrations. Since this attractant can be oxidized by myeloperoxidase from stimulated neutrophils, we determined if its efficacy was increased by protection from oxidation. Efficacy was increased by reducing agents and by molecules with oxidizable sulfur, 10-5M methionine or 1.5×10-6M albumin. The antioxidant effect was on the attractant, not the cells: albumin in the cell compartment did not increase responses. Furthermore, antioxidants had no effect on efficacy of fNle-Leu-Phe-Nle-Tyr-Lys, an attractant that also stimulated superoxide release but had no oxidizable sulfur.",

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T2 - Oxidation of stimulus by responding cells

AU - Yoshimura, Teizo

AU - Rot, Antal

AU - Leonard, Edward J.

PY - 1986/7/16

Y1 - 1986/7/16

N2 - Although all human neutrophils have receptors for formyl-methionyl-leucyl-phenylalanine, only 20-30% migrate in vitro at optimal attractant concentrations. Since this attractant can be oxidized by myeloperoxidase from stimulated neutrophils, we determined if its efficacy was increased by protection from oxidation. Efficacy was increased by reducing agents and by molecules with oxidizable sulfur, 10-5M methionine or 1.5×10-6M albumin. The antioxidant effect was on the attractant, not the cells: albumin in the cell compartment did not increase responses. Furthermore, antioxidants had no effect on efficacy of fNle-Leu-Phe-Nle-Tyr-Lys, an attractant that also stimulated superoxide release but had no oxidizable sulfur.

AB - Although all human neutrophils have receptors for formyl-methionyl-leucyl-phenylalanine, only 20-30% migrate in vitro at optimal attractant concentrations. Since this attractant can be oxidized by myeloperoxidase from stimulated neutrophils, we determined if its efficacy was increased by protection from oxidation. Efficacy was increased by reducing agents and by molecules with oxidizable sulfur, 10-5M methionine or 1.5×10-6M albumin. The antioxidant effect was on the attractant, not the cells: albumin in the cell compartment did not increase responses. Furthermore, antioxidants had no effect on efficacy of fNle-Leu-Phe-Nle-Tyr-Lys, an attractant that also stimulated superoxide release but had no oxidizable sulfur.

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Chemoattractant efficacy: Oxidation of stimulus by responding cells

Abstract

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