Clonal analysis of human T-cell responses to fractionated house dust mite antigens (Dermatophagoides pteronyssinus)

Mitsuhiro Okano, Toshiaki Nagano, Toshiro Ono, Yu Masuda, Nobuo Ohta

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


In the present study, we investigated the cellular basis of house dust mitedriven immune responsiveness in an atopic individual with perennial rhinitis. We established 40 human T-cell clones (CD3+, 4+, 8-) reactive to Dermatophagoides pteronyssinus (Dp) antigen under the restriction of HLA-DR. By using the crude Dp antigen and its 14 molecular weight (MW) fractions, we analyzed the distribution of T-cell-recognizing sites in the whole Dp extract. We tested T-cell-mediated immunity through two parameters; the identification of Dp fractions inducing T-cell proliferation, and the ability of T-cell clones to secrete IL-2, IL-4, and IFN-γ. According to a prominent peak among fraction-driven T-cell proliferation, we observed that T-cell clones that recognized 45,000-to 95,000-MW fractions were common, while clones reactive to 15,000-to 25,000-MW fractions were less frequent. Several clones were also reactive to antigens of Dermatophagoides farinae or other insects. Based on the responses of cloned T cells, we observed at least 9 distinct T epitopes in crude Dp antigen. These T-cell clones had a heterogenous secretory pattern of cytokines. T-cell clones showed no association between their ability to produce regulatory cytokine and their recognition of particular Dp fractions.

Original languageEnglish
Pages (from-to)82-88
Number of pages7
JournalInternational archives of allergy and immunology
Issue number1
Publication statusPublished - Jan 1 1993


  • Cytokine
  • House dust mite allergy
  • T epitope
  • T-cell clone

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


Dive into the research topics of 'Clonal analysis of human T-cell responses to fractionated house dust mite antigens (Dermatophagoides pteronyssinus)'. Together they form a unique fingerprint.

Cite this