TY - JOUR
T1 - Cloning of Cyc (Bmal1) homolog in Bombyx mori
T2 - Structural analysis and tissue specific distributions
AU - Markova, Elitza P.
AU - Ueda, Hitoshi
AU - Sakamoto, Katsuhiko
AU - Oishi, Katsutaka
AU - Shimada, Toru
AU - Takeda, Makio
N1 - Funding Information:
This study was supported by ‘Research for Future’ program from JSPS (99L01205) and by a grant-in-aid for scientific research from the Ministry of Science, Sport and Culture of Japan (11460021).
PY - 2003/3/1
Y1 - 2003/3/1
N2 - Cycle (Bmal1) is one of the circadian clock genes and the key regulator of the circadian system in many organisms, encoding a bHLH-PAS transcription factor. In the present study, we cloned cycle homolog (BmCyc) in Bombyx mori. We performed polymerase chain reaction with degenerated primers deduced from the conserved amino acid sequences of mammalian BMAL1 and Drosophila CYCLE. Then the partial clone obtained was used as a probe for screening a cDNA library constructed from pupal brains of B. mori. BmCyc is 5703 nucleotides long and encodes 700 amino acid residues. The BmCYC has bHLH, PAS A and PAS B domains, and the sequence identities for these domains are 85, 60 and 50%, to Drosophila CYCLE (dCYC) and 69, 58 and 50%, to human BMAL1 (hBMAL1), respectively. The deduced amino acid sequence of BmCYC is 37% identical to that of dCYC and 28% to hBMAL1. Northern blot analysis demonstrates that BmCyc gene was expressed in all the tissues tested, which were head, fat body, silk glands, and midgut. Also no significant day, night time-specific difference in expression of BmCyc gene in the head was detected.
AB - Cycle (Bmal1) is one of the circadian clock genes and the key regulator of the circadian system in many organisms, encoding a bHLH-PAS transcription factor. In the present study, we cloned cycle homolog (BmCyc) in Bombyx mori. We performed polymerase chain reaction with degenerated primers deduced from the conserved amino acid sequences of mammalian BMAL1 and Drosophila CYCLE. Then the partial clone obtained was used as a probe for screening a cDNA library constructed from pupal brains of B. mori. BmCyc is 5703 nucleotides long and encodes 700 amino acid residues. The BmCYC has bHLH, PAS A and PAS B domains, and the sequence identities for these domains are 85, 60 and 50%, to Drosophila CYCLE (dCYC) and 69, 58 and 50%, to human BMAL1 (hBMAL1), respectively. The deduced amino acid sequence of BmCYC is 37% identical to that of dCYC and 28% to hBMAL1. Northern blot analysis demonstrates that BmCyc gene was expressed in all the tissues tested, which were head, fat body, silk glands, and midgut. Also no significant day, night time-specific difference in expression of BmCyc gene in the head was detected.
KW - BMAL1
KW - Bombyx mori
KW - Circadian rhythm
KW - Clock genes
KW - Cloning
KW - Cycle gene
KW - Gene expression
KW - bHLH-PAS
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U2 - 10.1016/S1096-4959(03)00004-6
DO - 10.1016/S1096-4959(03)00004-6
M3 - Article
C2 - 12628385
AN - SCOPUS:0037333269
SN - 0305-0491
VL - 134
SP - 535
EP - 542
JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
IS - 3
ER -