TY - JOUR
T1 - Cloning of two members of the calcitonin-family receptors from stingray, Dasyatis akajei
T2 - Possible physiological roles of the calcitonin family in osmoregulation
AU - Suzuki, Nobuo
AU - Sekiguchi, Toshio
AU - Satake, Honoo
AU - Kato, Kanoko
AU - Nishiyama, Yudai
AU - Takahashi, Hideya
AU - Danks, Janine A.
AU - Martin, T. John
AU - Hattori, Atsuhiko
AU - Nakano, Masaki
AU - Kakikawa, Makiko
AU - Yamada, Sotoshi
AU - Ogoshi, Maho
AU - Hyodo, Susumu
AU - Yamaguchi, Yoko
AU - Chowdhury, Vishwajit S.
AU - Hayakawa, Kazuichi
AU - Funahashi, Hisayuki
AU - Sakamoto, Tatsuya
AU - Sasayama, Yuichi
N1 - Funding Information:
This study was supported in part by grants to N.S. ( Grant-in-Aid for Scientific Research (C) No. 21500404 by JSPS; Grant-in-Aid for Space Utilization by Japan Aerospace Exploration Agency), to A.H. ( Grant-in-Aid for Scientific Research (C) No. 21570062 by JSPS), to T.S. ( Grant-in-Aid for Scientific Research (C) No. 22570065 by JSPS), to M.O. ( Grant-in-Aid for Encouragement of Scientists No. 22870020 by JSPS), and to K.H. [the Environment Research and Technology Development Fund (B-0905) by the Ministry of the Environment in Japan; Health and Labor Sciences Research Grants of Ministry of Health, Labor and Welfare, Japan; Grant-in-Aid for Scientific Research (B) No. 21390034 by JSPS]. This work was carried out in part as a joint-research in Japanese Association for Marine Biology (JAMBIO).
PY - 2012/5/15
Y1 - 2012/5/15
N2 - In cartilaginous fish, two cDNAs encoding calcitonin-family receptors were isolated for the first time from the stingray brain. The open reading frame of one receptor cDNA coded a 525-amino acid protein. The amino acid identity of this receptor to human calcitonin-receptor-like receptor (CRLR) is 64.5%, frog CRLR is 64.7%, and flounder CRLR is 61.2% and this was higher than to human calcitonin receptor (CTR) (46.1%), frog CTR (54.7%), and flounder CTR (48.9%). We strongly suggested that this receptor is a ray CRLR based on phylogenetic analysis. In case of the second receptor, amino acid identity among CRLRs (human 50.5%, frog 50.7%, flounder 48.0%) and CTRs (human 43.2%, frog 49.1%, flounder 41.8%) was similar. From phylogenetic analysis of both CRLRs and CTRs, we believe that this receptor is ray CTR. The expression of ray CRLR mRNA was predominantly detected in the nervous system (brain) and vascular system (atrium, ventricle, and gill), which reflects the similar localization of CGRP in the nervous and vascular systems as mammals. It was observed that the second receptor was expressed in several tissues, namely cartilage, brain, pituitary gland, gill, atrium, ventricle, pancreas, spleen, liver, gall bladder, intestine, rectal gland, kidney, testis and ovary. This localization pattern was very similar to flounder CTR. Both receptor mRNAs were strongly expressed in the gill. This suggests that the calcitonin-family members are involved in the osmoregulation of stingray as this fish is known to be euryhaline. When a stingray was transferred to diluted seawater (20% seawater), the expression of both receptors significantly decreased in the gill. Similar results were obtained in the kidney of the stingray. Thus, our cloning and isolation of both receptors in the stingray will be helpful for elucidation of their physiological role(s) such as osmoregulation including calcium metabolism of cartilaginous fish.
AB - In cartilaginous fish, two cDNAs encoding calcitonin-family receptors were isolated for the first time from the stingray brain. The open reading frame of one receptor cDNA coded a 525-amino acid protein. The amino acid identity of this receptor to human calcitonin-receptor-like receptor (CRLR) is 64.5%, frog CRLR is 64.7%, and flounder CRLR is 61.2% and this was higher than to human calcitonin receptor (CTR) (46.1%), frog CTR (54.7%), and flounder CTR (48.9%). We strongly suggested that this receptor is a ray CRLR based on phylogenetic analysis. In case of the second receptor, amino acid identity among CRLRs (human 50.5%, frog 50.7%, flounder 48.0%) and CTRs (human 43.2%, frog 49.1%, flounder 41.8%) was similar. From phylogenetic analysis of both CRLRs and CTRs, we believe that this receptor is ray CTR. The expression of ray CRLR mRNA was predominantly detected in the nervous system (brain) and vascular system (atrium, ventricle, and gill), which reflects the similar localization of CGRP in the nervous and vascular systems as mammals. It was observed that the second receptor was expressed in several tissues, namely cartilage, brain, pituitary gland, gill, atrium, ventricle, pancreas, spleen, liver, gall bladder, intestine, rectal gland, kidney, testis and ovary. This localization pattern was very similar to flounder CTR. Both receptor mRNAs were strongly expressed in the gill. This suggests that the calcitonin-family members are involved in the osmoregulation of stingray as this fish is known to be euryhaline. When a stingray was transferred to diluted seawater (20% seawater), the expression of both receptors significantly decreased in the gill. Similar results were obtained in the kidney of the stingray. Thus, our cloning and isolation of both receptors in the stingray will be helpful for elucidation of their physiological role(s) such as osmoregulation including calcium metabolism of cartilaginous fish.
KW - Calcitonin
KW - Calcitonin gene-related peptide
KW - Elasmobranch
KW - Tissue expression
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U2 - 10.1016/j.gene.2012.03.042
DO - 10.1016/j.gene.2012.03.042
M3 - Article
C2 - 22429839
AN - SCOPUS:84860237348
SN - 0378-1119
VL - 499
SP - 326
EP - 331
JO - Gene
JF - Gene
IS - 2
ER -