Collagen type IV-specific tripeptides for selective adhesion of endothelial and smooth muscle cells

Kei Kanie, Yuji Narita, Yingzi Zhao, Fumiaki Kuwabara, Makoto Satake, Susumu Honda, Hiroaki Kaneko, Tomohiko Yoshioka, Mina Okochi, Hiroyuki Honda, Ryuji Kato

Research output: Contribution to journalArticlepeer-review

63 Citations (Scopus)


Controlling the balance of endothelial cells (ECs) and smooth muscle cells (SMCs) in blood vessels is critically important to minimize the risk associated with vascular implants. Extracellular matrix (ECM) plays a key role in controlling the cellular balance, suggesting a promising source of cell-selective peptides. To obtain EC- or SMC-selective peptides, we start by highlighting sequence differences found among ECM molecules as enriched targets for cell-selective peptides. We explored the EC- or SMC-selective performance of tripeptides that are specifically enriched only in collagen type IV, but not in types I, II, III, and V. Collagen type IV was chosen since it is the major ECM in the basement membrane of blood vessels, which separates ECs and SMCs. Among 114 collagen type IV-derived tripeptides pre-screened from in silico analysis, 22 peptides (19%) were found to promote cell-selective adhesion, as determined by peptide array. One of the best performing EC-selective peptides (Cys-Ala-Gly (CAG)) was mixed into an electrospun fine-fiber, a vascular graft material, for practical application. Compared to unmodified fiber, the CAG containing fiber surface was found to enhance adhesion of ECs (+190%) while limiting SMCs (-20%). These results are not only consistent with the hypothesis of ECM as a source of cell selective peptides, but also suggest a new genre of EC- or SMC-selective peptides for tissue engineering applications. Collectively, these findings favorably support the screening approach used to discover new peptides for these purposes.

Original languageEnglish
Pages (from-to)1808-1816
Number of pages9
JournalBiotechnology and Bioengineering
Issue number7
Publication statusPublished - Jul 2012
Externally publishedYes


  • Cell selective peptide
  • Collagen
  • Endothelial cell
  • Extracellular matrix (ECM)
  • Smooth muscle cell

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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