Combined apoptotic effects of peptide and miRNA in a peptide/miRNA nanocomplex

Hyungjin Kim; Mizuki Kitamatsu; Takashi Ohtsuki

doi:10.1016/j.jbiosc.2019.01.003

Combined apoptotic effects of peptide and miRNA in a peptide/miRNA nanocomplex

Hyungjin Kim, Mizuki Kitamatsu, Takashi Ohtsuki

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The present study investigated combined biological effects of peptide and miRNA in a peptide/miRNA nanocomplex. We utilized TatBim peptide as a cell-penetrating peptide-based RNA carrier with apoptotic activity. miRNA with apoptotic activity (miR-34a) was used for complex formation to investigate the additional effects of the combination with TatBim peptide. TatBim peptide and the miRNA formed nanocomplexes (approximately 250 nm in diameter), and these complexes were efficiently internalized by cells. Despite its efficient cell internalization, apoptotic activity of the nanocomplex decreased with increasing RNA content. However, photosensitizer-attachment to TatBim and photoirradiation significantly improved the apoptotic activity of the nanocomplex by facilitating dispersion of the peptide and RNA in the cytoplasm. Combined apoptotic activity of both TatBim peptide and miR-34a in the nanocomplex was demonstrated by substituting TatBim with Lipofectamine and by substituting miR-34a with scrambled siRNA.

Original language	English
Pages (from-to)	110-116
Number of pages	7
Journal	Journal of Bioscience and Bioengineering
Volume	128
Issue number	1
DOIs	https://doi.org/10.1016/j.jbiosc.2019.01.003
Publication status	Published - Jul 2019

Keywords

Apoptosis
Cell-penetrating peptide
Nanocomplex
Photosensitizer
TatBim
miRNA

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1016/j.jbiosc.2019.01.003

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title = "Combined apoptotic effects of peptide and miRNA in a peptide/miRNA nanocomplex",

abstract = "The present study investigated combined biological effects of peptide and miRNA in a peptide/miRNA nanocomplex. We utilized TatBim peptide as a cell-penetrating peptide-based RNA carrier with apoptotic activity. miRNA with apoptotic activity (miR-34a) was used for complex formation to investigate the additional effects of the combination with TatBim peptide. TatBim peptide and the miRNA formed nanocomplexes (approximately 250 nm in diameter), and these complexes were efficiently internalized by cells. Despite its efficient cell internalization, apoptotic activity of the nanocomplex decreased with increasing RNA content. However, photosensitizer-attachment to TatBim and photoirradiation significantly improved the apoptotic activity of the nanocomplex by facilitating dispersion of the peptide and RNA in the cytoplasm. Combined apoptotic activity of both TatBim peptide and miR-34a in the nanocomplex was demonstrated by substituting TatBim with Lipofectamine and by substituting miR-34a with scrambled siRNA.",

keywords = "Apoptosis, Cell-penetrating peptide, Nanocomplex, Photosensitizer, TatBim, miRNA",

author = "Hyungjin Kim and Mizuki Kitamatsu and Takashi Ohtsuki",

note = "Funding Information: We thank Dr. K. Watanabe (Okayama University) for the preparation of the RNA, Dr. T. Uchida and Ms. M. Yoneda (Okayama University) for their support in the STEM and EDS analyses, and Prof. T. Ono (Okayama University) for his support in the Dynamic light scattering analyses. This work was supported by Okayama Foundation for Science and Technology. Funding Information: We thank Dr. K. Watanabe (Okayama University) for the preparation of the RNA, Dr. T. Uchida and Ms. M. Yoneda (Okayama University) for their support in the STEM and EDS analyses, and Prof. T. Ono (Okayama University) for his support in the Dynamic light scattering analyses. This work was supported by Okayama Foundation for Science and Technology . Publisher Copyright: {\textcopyright} 2019 The Society for Biotechnology, Japan",

year = "2019",

month = jul,

doi = "10.1016/j.jbiosc.2019.01.003",

language = "English",

volume = "128",

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journal = "Journal of Bioscience and Bioengineering",

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publisher = "The Society for Biotechnology, Japan",

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AU - Kim, Hyungjin

AU - Kitamatsu, Mizuki

AU - Ohtsuki, Takashi

N1 - Funding Information: We thank Dr. K. Watanabe (Okayama University) for the preparation of the RNA, Dr. T. Uchida and Ms. M. Yoneda (Okayama University) for their support in the STEM and EDS analyses, and Prof. T. Ono (Okayama University) for his support in the Dynamic light scattering analyses. This work was supported by Okayama Foundation for Science and Technology. Funding Information: We thank Dr. K. Watanabe (Okayama University) for the preparation of the RNA, Dr. T. Uchida and Ms. M. Yoneda (Okayama University) for their support in the STEM and EDS analyses, and Prof. T. Ono (Okayama University) for his support in the Dynamic light scattering analyses. This work was supported by Okayama Foundation for Science and Technology . Publisher Copyright: © 2019 The Society for Biotechnology, Japan

PY - 2019/7

Y1 - 2019/7

N2 - The present study investigated combined biological effects of peptide and miRNA in a peptide/miRNA nanocomplex. We utilized TatBim peptide as a cell-penetrating peptide-based RNA carrier with apoptotic activity. miRNA with apoptotic activity (miR-34a) was used for complex formation to investigate the additional effects of the combination with TatBim peptide. TatBim peptide and the miRNA formed nanocomplexes (approximately 250 nm in diameter), and these complexes were efficiently internalized by cells. Despite its efficient cell internalization, apoptotic activity of the nanocomplex decreased with increasing RNA content. However, photosensitizer-attachment to TatBim and photoirradiation significantly improved the apoptotic activity of the nanocomplex by facilitating dispersion of the peptide and RNA in the cytoplasm. Combined apoptotic activity of both TatBim peptide and miR-34a in the nanocomplex was demonstrated by substituting TatBim with Lipofectamine and by substituting miR-34a with scrambled siRNA.

AB - The present study investigated combined biological effects of peptide and miRNA in a peptide/miRNA nanocomplex. We utilized TatBim peptide as a cell-penetrating peptide-based RNA carrier with apoptotic activity. miRNA with apoptotic activity (miR-34a) was used for complex formation to investigate the additional effects of the combination with TatBim peptide. TatBim peptide and the miRNA formed nanocomplexes (approximately 250 nm in diameter), and these complexes were efficiently internalized by cells. Despite its efficient cell internalization, apoptotic activity of the nanocomplex decreased with increasing RNA content. However, photosensitizer-attachment to TatBim and photoirradiation significantly improved the apoptotic activity of the nanocomplex by facilitating dispersion of the peptide and RNA in the cytoplasm. Combined apoptotic activity of both TatBim peptide and miR-34a in the nanocomplex was demonstrated by substituting TatBim with Lipofectamine and by substituting miR-34a with scrambled siRNA.

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Combined apoptotic effects of peptide and miRNA in a peptide/miRNA nanocomplex

Abstract

Keywords

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