Comparative analysis of flagellin glycans among pathovars of phytopathogenic Pseudomonas syringae

Kazuhiro Chiku, Masanobu Yamamoto, Mayumi Ohnishi-Kameyama, Tadashi Ishii, Mitsuru Yoshida, Fumiko Taguchi, Yuki Ichinose, Hiroshi Ono

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


Flagellin is a principal component of the flagellum filament. Previously, we reported that the flagellin of Pseudomonas syringae pv. tabaci 6605 (Pta6605) was glycosylated by oligosaccharides composed of two or three l-rhamnosyl (l-Rha) residues and a terminal 4,6-dideoxy-4-(3-hydroxybutanamide)-2-O- methylglucopyranosyl residue. In this study, we characterized the chemical structure of flagellin glycans in P. syringae pathovars glycinea race 4 (Pgl4), phaseolicola 1448A (Pph1448A), tomato DC3000 (PtoDC3000), and syringae B728a (PsyB728a). Flagellin glycans were released by hydrazinolysis, labeled on the reducing ends with 2-aminopyridine (PA), and the PA-labeled oligosaccharides were isolated by high-performance liquid chromatography. The purified PA-labeled glycans were analyzed by mass spectrometry and NMR spectroscopy. The results showed that the glycans on flagellin of Pgl4, PtoDC3000, and Pph1448A were identical to those of Pta6605, which were characterized previously. The flagellin of PsyB728a is O-glycosylated with a novel trisaccharide identified as 2-acetamide-2-deoxy-β-d-glucopyranosyl-(1→2)-3-O-methyl-α-l- rhamnopyranosyl-(1→2)-l-rhamnose. Our data indicate that flagellin glycosylation of P. syringae pathovars has universality with little diversity.

Original languageEnglish
Pages (from-to)100-104
Number of pages5
JournalCarbohydrate Research
Publication statusPublished - 2013


  • 2-Aminopyridine
  • Flagellin glycan
  • Glycoprotein
  • NMR
  • Pseudomonas syringae

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry


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