Comparison of the long-term effects on rabbit bone defects between Tetrabone® and β-tricalcium phosphate granules implantation

Sungjin Choi, I. Li Liu, Kenichi Yamamoto, Muneki Honnami, Shinsuke Ohba, Ryosuke Echigo, Takamasa Sakai, Kazuyo Igawa, Shigeki Suzuki, Ryouhei Nishimura, Ung il Chung, Nobuo Sasaki, Manabu Mochizuki

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Tetrabone® is a newly developed granular artificial bone. The 1-mm Tetrabone® has a four-legged structure. In this study, the long-term effect of implanting Tetrabone® or β-TCP granules in rabbit femoral cylindrical defects was evaluated. The rabbits were euthanized at 4, 13, and 26 weeks after implantation. Micro-CT was conducted to evaluate the residual material volume and the non-osseous tissue volume. New bone tissue areas were measured by histological analysis. Micro-CT imaging showed that the residual material volume in the β-TCP group had decreased significantly at 4 weeks after implantation (P < 0.05) and that the β-TCP granules had nearly disappeared at 26 weeks after implantation. In the Tetrabone® group, it did not significantly change until 13 weeks after implantation; it then continued to decrease slightly until 26 weeks after implantation. The non-osseous volume increased in the β-TCP group, whereas that of the Tetrabone® group decreased (P < 0.05). Histological examination showed that the new bone areas were significantly greater in the Tetrabone® group than in the β-TCP group at 13 and 26 weeks. In conclusion, resorption of β-TCP granules occurs before sufficient bone formation, thereby allowing non-osseous tissue invasion. Tetrabone® resorption progressed slowly while the new bone tissues were formed, thus allowing better healing. Tetrabone® showed better osteoconductivity, whereas the β-TCP granules lost their function over a long duration. These results may be caused by the differences in the absorption rate of the granules, intergranular pore structure, and crystallinity of each granule.

Original languageEnglish
Pages (from-to)344-351
Number of pages8
JournalJournal of Artificial Organs
Issue number4
Publication statusPublished - Dec 5 2014
Externally publishedYes


  • Artificial bones
  • OCP
  • Rabbit
  • Tetrabone
  • β-TCP granules

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering
  • Cardiology and Cardiovascular Medicine


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