Competitive adsorption of fibronectin and albumin on hydroxyapatite nanocrystals

Motohiro Tagaya, Toshiyuki Ikoma, Nobutaka Hanagata, Tomohiko Yoshioka, Junzo Tanaka

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)


Competitive adsorption of two-component solutions containing fibronectin (Fn) and albumin (Ab) on hydroxyapatite (HAp) nanocrystals was analyzed in situ using the quartz crystal microbalance with dissipation (QCM-D) technique. Adsorption of the one-component protein (Fn or Ab) and the two-component proteins adjusted to different molar ratios of Fn to Ab at a fixed Fn concentration was investigated. The frequency shift (Δf; Hz) and the dissipation energy shift (ΔD) were measured with the QCM-D technique, and the viscoelastic changes of adlayers were evaluated by the saturated ΔD/Δf value and the Voigt-based viscoelastic model. For the adsorption of the one-component protein, the Fn adlayer showed a larger mass and higher viscoelasticity than the Ab adlayer, indicating the higher affinity of Fn on HAp. For the adsorption of the two-component proteins, the viscoelastic properties of the adlayers became elastic with increase in Ab concentration, whereas the adsorption mass was similar to that of Fn in the one-component solution regardless of the Ab concentration. The specific binding mass of the Ab antibody to the adlayers increased with increase in Ab concentration, whereas that of the Fn antibody decreased. Therefore, Fn preferentially adsorbs on HAp and Ab subsequently interacts with the adlayers, indicating that the interfacial viscoelasticity of the adlayers was dominated by the interaction between Fn and Ab.

Original languageEnglish
Article number034411
JournalScience and Technology of Advanced Materials
Issue number3
Publication statusPublished - Jun 2011
Externally publishedYes


  • QCM-D
  • competitive adsorption
  • hydroxyapatite
  • soft interface
  • viscoelastic property

ASJC Scopus subject areas

  • General Materials Science


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