TY - JOUR
T1 - Complete genome sequences of rat and mouse segmented filamentous bacteria, a potent inducer of Th17 cell differentiation
AU - Prakash, Tulika
AU - Oshima, Kenshiro
AU - Morita, Hidetoshi
AU - Fukuda, Shinji
AU - Imaoka, Akemi
AU - Kumar, Naveen
AU - Sharma, Vineet K.
AU - Kim, Seok Won
AU - Takahashi, Mahoko
AU - Saitou, Naruya
AU - Taylor, Todd D.
AU - Ohno, Hiroshi
AU - Umesaki, Yoshinori
AU - Hattori, Masahira
N1 - Funding Information:
We thank K. Ueda and T. Beppu (Nihon University) for discussion about the SEM and TEM images of SFB and K. Furuya, C. Shindo, H. Inaba, E. Iioka, M. Yoshizaki, Y. Takayama, E. Ohmori, A. Nakano, and Y. Hattori for technical support. We thank D.R. Littman and I.I. Ivanov for sharing information about their analysis of the mouse SFB genome prior to publication. This research was supported in part by grants from the Ministry of Education, Culture, Sports, Science, and Technology of Japan: a Grant-in-Aid for Scientific Research on Priority Areas “Comprehensive Genomics” (M.H.), “Membrane Traffic” (H.O.), Young Scientists (S.F.), Scientific Research (H.O.), Scientific Research on Innovative Areas “Intracellular Logistics” (H.O.), and the global COE project of “Genome Information Big Bang” (M.H. and K.O.). This work was also supported in part by a RIKEN DRI Research Grant (S.F.), the Naito Foundation (S.F.), the Sasakawa Scientific Research Grant from the Japan Science Society (S.F.), and the Private University Scientific Foundation (H.M.).
PY - 2011/9/15
Y1 - 2011/9/15
N2 - Segmented filamentous bacteria (SFB) are noncultivable commensals inhabiting the gut of various vertebrate species and have been shown to induce Th17 cells in mice. We present the complete genome sequences of both rat and mouse SFB isolated from SFB-monocolonized hosts. The rat and mouse SFB genomes each harbor a single circular chromosome of 1.52 and 1.59 Mb encoding 1346 and 1420 protein-coding genes, respectively. The overall nucleotide identity between the two genomes is 86%, and the substitution rate was estimated to be similar to that of the free-living E. coli. SFB genomes encode typical genes for anaerobic fermentation and spore and flagella formation, but lack most of the amino acid biosynthesis enzymes, reminiscent of pathogenic Clostridia, exhibiting large dependency on the host. However, SFB lack most of the clostridial virulence-related genes. Comparative analysis with clostridial genomes suggested possible mechanisms for host responses and specific adaptations in the intestine.
AB - Segmented filamentous bacteria (SFB) are noncultivable commensals inhabiting the gut of various vertebrate species and have been shown to induce Th17 cells in mice. We present the complete genome sequences of both rat and mouse SFB isolated from SFB-monocolonized hosts. The rat and mouse SFB genomes each harbor a single circular chromosome of 1.52 and 1.59 Mb encoding 1346 and 1420 protein-coding genes, respectively. The overall nucleotide identity between the two genomes is 86%, and the substitution rate was estimated to be similar to that of the free-living E. coli. SFB genomes encode typical genes for anaerobic fermentation and spore and flagella formation, but lack most of the amino acid biosynthesis enzymes, reminiscent of pathogenic Clostridia, exhibiting large dependency on the host. However, SFB lack most of the clostridial virulence-related genes. Comparative analysis with clostridial genomes suggested possible mechanisms for host responses and specific adaptations in the intestine.
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U2 - 10.1016/j.chom.2011.08.007
DO - 10.1016/j.chom.2011.08.007
M3 - Article
C2 - 21925114
AN - SCOPUS:80053045987
SN - 1931-3128
VL - 10
SP - 273
EP - 284
JO - Cell Host and Microbe
JF - Cell Host and Microbe
IS - 3
ER -