TY - JOUR
T1 - Construction and characterization of the PGN_0296 mutant of Porphyromonas gingivalis
AU - Shahriar, Abu Saleh Muhammad
AU - Ono, Shintaro
AU - Nakayama, Masaaki
AU - Ohara, Naoko
AU - Ohara, Naoya
N1 - Funding Information:
This study was supported by JSPS KAKENHI Grant Numbers 17H04378 (for N.O.), 26861573 and 17K11668 (for M.N.), and 20K09939 (for S.O.).
Publisher Copyright:
© 2020 Japanese Association for Oral Biology
PY - 2020/12
Y1 - 2020/12
N2 - The periodontal pathogen Porphyromonas gingivalis produces gingipains (Kgp, RgpA, and RgpB), cysteine proteases involved in the organism's virulence, and pigmentation. We previously showed that deletion of the PGN_0297 and PGN_0300 genes reduced the proteolytic activity of gingipains. The role of the PGN_0296 gene, consisting of an operon with the PGN_0297 and PGN_0300 genes, is unclear. Herein, we examined the effect of PGN_0296 gene deletion on the proteolytic activity. Although the proteolytic activity of the gingipains did not decrease in the culture supernatant of a PGN_0296 gene deletion mutant (ΔPGN_0296), the growth was delayed.
AB - The periodontal pathogen Porphyromonas gingivalis produces gingipains (Kgp, RgpA, and RgpB), cysteine proteases involved in the organism's virulence, and pigmentation. We previously showed that deletion of the PGN_0297 and PGN_0300 genes reduced the proteolytic activity of gingipains. The role of the PGN_0296 gene, consisting of an operon with the PGN_0297 and PGN_0300 genes, is unclear. Herein, we examined the effect of PGN_0296 gene deletion on the proteolytic activity. Although the proteolytic activity of the gingipains did not decrease in the culture supernatant of a PGN_0296 gene deletion mutant (ΔPGN_0296), the growth was delayed.
KW - Bacterial secretion system
KW - Gingipain
KW - Periodontal diseases
KW - Porphyromonas gingivalis
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U2 - 10.1016/j.job.2020.09.007
DO - 10.1016/j.job.2020.09.007
M3 - Article
C2 - 33038516
AN - SCOPUS:85092705375
SN - 1349-0079
VL - 62
SP - 322
EP - 326
JO - Journal of Oral Biosciences
JF - Journal of Oral Biosciences
IS - 4
ER -