Construction of a series of mutants lacking all of the four major mex operons for multidrug efflux pumps or possessing each one of the operons from Pseudomonas aeruginosa PAO1: MexCD-OprJ is an inducible pump

Yuji Morita; Yukiko Komori; Takehiko Mima; Teruo Kuroda; Tohru Mizushima; Tomofusa Tsuchiya

doi:10.1016/S0378-1097(01)00314-7

Construction of a series of mutants lacking all of the four major mex operons for multidrug efflux pumps or possessing each one of the operons from Pseudomonas aeruginosa PAO1: MexCD-OprJ is an inducible pump

Yuji Morita, Yukiko Komori, Takehiko Mima, Teruo Kuroda, Tohru Mizushima, Tomofusa Tsuchiya

Research output: Contribution to journal › Article › peer-review

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Abstract

We constructed a series of deletion mutants lacking all of the four major mex operons for Mex multidrug efflux pumps or possessing each one of the operons from Pseudomonas aeruginosa PAO1. The drug specificity of MexAB-OprM, MexXY-OprM and MexCD-OprJ was investigated. Surprisingly, we found that the MexCD-OprJ was an inducible pump, inducers of which were tetraphenylphosphonium chloride, ethidium bromide, rhodamine 6G and acriflavine. Fluoroquinolones, chloramphenicol, erythromycin and tetracycline were not inducers although they were substrates of MexCD-OprJ.

Original language	English
Pages (from-to)	139-143
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	202
Issue number	1
DOIs	https://doi.org/10.1016/S0378-1097(01)00314-7
Publication status	Published - Aug 7 2001

Keywords

A series of deletion mutants
Drug specificity
Mex multidrug efflux pump
MexCD-OprM
Pseudomonas aeruginosa

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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Morita, Y., Komori, Y., Mima, T., Kuroda, T., Mizushima, T., & Tsuchiya, T. (2001). Construction of a series of mutants lacking all of the four major mex operons for multidrug efflux pumps or possessing each one of the operons from Pseudomonas aeruginosa PAO1: MexCD-OprJ is an inducible pump. FEMS Microbiology Letters, 202(1), 139-143. https://doi.org/10.1016/S0378-1097(01)00314-7

Construction of a series of mutants lacking all of the four major mex operons for multidrug efflux pumps or possessing each one of the operons from Pseudomonas aeruginosa PAO1: MexCD-OprJ is an inducible pump. / Morita, Yuji; Komori, Yukiko; Mima, Takehiko et al.
In: FEMS Microbiology Letters, Vol. 202, No. 1, 07.08.2001, p. 139-143.

Research output: Contribution to journal › Article › peer-review

Morita, Y, Komori, Y, Mima, T, Kuroda, T, Mizushima, T & Tsuchiya, T 2001, 'Construction of a series of mutants lacking all of the four major mex operons for multidrug efflux pumps or possessing each one of the operons from Pseudomonas aeruginosa PAO1: MexCD-OprJ is an inducible pump', FEMS Microbiology Letters, vol. 202, no. 1, pp. 139-143. https://doi.org/10.1016/S0378-1097(01)00314-7

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abstract = "We constructed a series of deletion mutants lacking all of the four major mex operons for Mex multidrug efflux pumps or possessing each one of the operons from Pseudomonas aeruginosa PAO1. The drug specificity of MexAB-OprM, MexXY-OprM and MexCD-OprJ was investigated. Surprisingly, we found that the MexCD-OprJ was an inducible pump, inducers of which were tetraphenylphosphonium chloride, ethidium bromide, rhodamine 6G and acriflavine. Fluoroquinolones, chloramphenicol, erythromycin and tetracycline were not inducers although they were substrates of MexCD-OprJ.",

keywords = "A series of deletion mutants, Drug specificity, Mex multidrug efflux pump, MexCD-OprM, Pseudomonas aeruginosa",

author = "Yuji Morita and Yukiko Komori and Takehiko Mima and Teruo Kuroda and Tohru Mizushima and Tomofusa Tsuchiya",

note = "Funding Information: We thank Dr. H.P. Schweizer of Colorado State University for some bacterial strains, Dr. T. Nakae of Tokai University School of Medicine for plasmid pUC19- mexEF-oprN , and Dr. M. Varela of Eastern New Mexico University for critical reading of the manuscript. This research was supported by a grant from the Ministry of Education, Science, Sport and Culture of Japan. ",

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AU - Komori, Yukiko

AU - Mima, Takehiko

AU - Kuroda, Teruo

AU - Mizushima, Tohru

AU - Tsuchiya, Tomofusa

N1 - Funding Information: We thank Dr. H.P. Schweizer of Colorado State University for some bacterial strains, Dr. T. Nakae of Tokai University School of Medicine for plasmid pUC19- mexEF-oprN , and Dr. M. Varela of Eastern New Mexico University for critical reading of the manuscript. This research was supported by a grant from the Ministry of Education, Science, Sport and Culture of Japan.

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N2 - We constructed a series of deletion mutants lacking all of the four major mex operons for Mex multidrug efflux pumps or possessing each one of the operons from Pseudomonas aeruginosa PAO1. The drug specificity of MexAB-OprM, MexXY-OprM and MexCD-OprJ was investigated. Surprisingly, we found that the MexCD-OprJ was an inducible pump, inducers of which were tetraphenylphosphonium chloride, ethidium bromide, rhodamine 6G and acriflavine. Fluoroquinolones, chloramphenicol, erythromycin and tetracycline were not inducers although they were substrates of MexCD-OprJ.

AB - We constructed a series of deletion mutants lacking all of the four major mex operons for Mex multidrug efflux pumps or possessing each one of the operons from Pseudomonas aeruginosa PAO1. The drug specificity of MexAB-OprM, MexXY-OprM and MexCD-OprJ was investigated. Surprisingly, we found that the MexCD-OprJ was an inducible pump, inducers of which were tetraphenylphosphonium chloride, ethidium bromide, rhodamine 6G and acriflavine. Fluoroquinolones, chloramphenicol, erythromycin and tetracycline were not inducers although they were substrates of MexCD-OprJ.

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KW - MexCD-OprM

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Construction of a series of mutants lacking all of the four major mex operons for multidrug efflux pumps or possessing each one of the operons from Pseudomonas aeruginosa PAO1: MexCD-OprJ is an inducible pump

Abstract

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