Construction of mouse-embryonic-cell-derived 3D pacemaker tissues by layer-by-layer nanofilm coating

Yuto Amano, Takuya Igarashi, Akihiro Nishiguchi, Michiya Matsusaki, Yukihiro Saito, Kazufumi Nakamura, Hiroshi Ito, Mitsuru Akashi

Research output: Contribution to journalArticlepeer-review


For the treatment of cardiac arrhythmia, electronic pacemakers are often employed. However, they have issues such as bio-incompatibility and battery limitations. Recently, the use of cells expressing hyperpolarization-activated cyclic nucleotide-gated 4 (HCN4) channels for use as pacemaker cells instead of electronic pacemakers has attracted increasing attention. However, the cell transplantation treatment was not sufficiently effective because of the low engraftment rate of the transplanted cells and the risk of inflammatory reactions. Here, in order to overcome these issues, we constructed 3D-pacemaker tissues composed of mouse-embryonic- cell-derived cardiomyocytes (mESC-CMs) in which the HCN4 gene had been introduced by the cell accumulation technique. The obtained tissues beat faster than control tissues and beats per minute (BPM) increased clearly with tissue thickness. This is the first report suggesting the relation between BPM and tissue thickness. Moreover, the pacemaker tissue could control the beating of the patched tissue.

Original languageEnglish
Pages (from-to)466-471
Number of pages6
Issue number5
Publication statusPublished - May 1 2016


  • Biomaterials
  • Cell adhesion
  • Layer-by-layer assembly
  • Thin films
  • Tissue engineering

ASJC Scopus subject areas

  • Biomaterials
  • Renewable Energy, Sustainability and the Environment
  • Energy Engineering and Power Technology
  • Materials Chemistry


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