TY - JOUR
T1 - Construction of plants resistant to TYLCV by using artificial zinc-finger proteins.
AU - Koshino-Kimura, Yoshihiro
AU - Takenaka, Kosuke
AU - Domoto, Fumiya
AU - Ohashi, Masayoshi
AU - Miyazaki, Toshihide
AU - Aoyama, Yasuhiro
AU - Sera, Takashi
N1 - Copyright:
This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine
PY - 2009
Y1 - 2009
N2 - Previously, we have demonstrated that plant DNA virus replication could be inhibited in Arabidopsis thaliana by using an artificial zinc-finger protein (AZP) and created AZP-based transgenic A. thaliana resistant to DNA virus infection. Here we apply the AZP technology to tomato yellow leaf curl virus (TYLCV) causing serious damage to an important agricultural crop, tomato. An AZP was designed to block binding of the TYLCV replication protein (Rep) to the replication origin. The designed AZP had much higher affinities towards the replication origin than did the Rep, and efficiently blocked Rep binding in vitro. The AZP gene was then introduced into a plant genome with the help of Agrobacterium tumefaciens to generate the transgenic plants. The current status of the construction of the AZP-expressing transgenic plants will be reported.
AB - Previously, we have demonstrated that plant DNA virus replication could be inhibited in Arabidopsis thaliana by using an artificial zinc-finger protein (AZP) and created AZP-based transgenic A. thaliana resistant to DNA virus infection. Here we apply the AZP technology to tomato yellow leaf curl virus (TYLCV) causing serious damage to an important agricultural crop, tomato. An AZP was designed to block binding of the TYLCV replication protein (Rep) to the replication origin. The designed AZP had much higher affinities towards the replication origin than did the Rep, and efficiently blocked Rep binding in vitro. The AZP gene was then introduced into a plant genome with the help of Agrobacterium tumefaciens to generate the transgenic plants. The current status of the construction of the AZP-expressing transgenic plants will be reported.
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U2 - 10.1093/nass/nrp141
DO - 10.1093/nass/nrp141
M3 - Article
C2 - 19749370
AN - SCOPUS:77952506470
SN - 1746-8272
SP - 281
EP - 282
JO - Nucleic acids symposium series (2004)
JF - Nucleic acids symposium series (2004)
IS - 53
ER -