Continuous preparation of bicelles using hydrodynamic focusing method for bicelle to vesicle transition

Sung Hak Choi, Bong Su Kang, Toshinori Shimanouchi, Keesung Kim, Ho Sup Jung

Research output: Contribution to journalLetterpeer-review

4 Citations (Scopus)


Bicelle is one of the most stable phospholipid assemblies, which has tremendous applications in the research areas for drug delivery or structural studies of membrane proteins owing to its bio-membrane mimicking characteristics and high thermal stability. However, the conventional preparation method for bicelle demands complicated manufacturing processes and a long time so that the continuous synthesis method of bicelle using microfluidic chip has been playing an important role to expand its feasibility. We verified the general availability of hydrodynamic focusing method with microfluidic chip for bicelle synthesis using various kinds of lipids which have a phase transition temperature ranged from − 2 to 41 °C. Bicelle can be formed only when the inside temperature of microfluidic chip was over the phase transition temperature. Moreover, the concentration condition for bicelle formation varied depending on the lipids. Furthermore, the transition process characteristics from bicelle to vesicle were analyzed by effective q-value, mixing time and dilution condition. We verified that the size of transition vesicles was controlled according to the effective q-value, mixing time, and temperature.

Original languageEnglish
Article number7
JournalMicro and Nano Systems Letters
Issue number1
Publication statusPublished - Dec 2021


  • Bicelle
  • Dilution method
  • Hydrodynamic focusing
  • Microfluidic chip
  • Vesicle

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering


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