TY - JOUR
T1 - Continuous preparation of bicelles using hydrodynamic focusing method for bicelle to vesicle transition
AU - Choi, Sung Hak
AU - Kang, Bong Su
AU - Shimanouchi, Toshinori
AU - Kim, Keesung
AU - Jung, Ho Sup
N1 - Funding Information:
This work was carried out with the support of "Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ01574703)" Rural Development Administration, Republic of Korea and was partially supported under the framework of international cooperation program managed by the National Research Foundation of Korea (NRF-2020K2A9A2A08000174) and Basic Research Program funded by the Korea Institute of Machinery and Materials (Grant Number: NK226E), Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through Agro and Livestock Products Safety Flow Management Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (118105-3).
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12
Y1 - 2021/12
N2 - Bicelle is one of the most stable phospholipid assemblies, which has tremendous applications in the research areas for drug delivery or structural studies of membrane proteins owing to its bio-membrane mimicking characteristics and high thermal stability. However, the conventional preparation method for bicelle demands complicated manufacturing processes and a long time so that the continuous synthesis method of bicelle using microfluidic chip has been playing an important role to expand its feasibility. We verified the general availability of hydrodynamic focusing method with microfluidic chip for bicelle synthesis using various kinds of lipids which have a phase transition temperature ranged from − 2 to 41 °C. Bicelle can be formed only when the inside temperature of microfluidic chip was over the phase transition temperature. Moreover, the concentration condition for bicelle formation varied depending on the lipids. Furthermore, the transition process characteristics from bicelle to vesicle were analyzed by effective q-value, mixing time and dilution condition. We verified that the size of transition vesicles was controlled according to the effective q-value, mixing time, and temperature.
AB - Bicelle is one of the most stable phospholipid assemblies, which has tremendous applications in the research areas for drug delivery or structural studies of membrane proteins owing to its bio-membrane mimicking characteristics and high thermal stability. However, the conventional preparation method for bicelle demands complicated manufacturing processes and a long time so that the continuous synthesis method of bicelle using microfluidic chip has been playing an important role to expand its feasibility. We verified the general availability of hydrodynamic focusing method with microfluidic chip for bicelle synthesis using various kinds of lipids which have a phase transition temperature ranged from − 2 to 41 °C. Bicelle can be formed only when the inside temperature of microfluidic chip was over the phase transition temperature. Moreover, the concentration condition for bicelle formation varied depending on the lipids. Furthermore, the transition process characteristics from bicelle to vesicle were analyzed by effective q-value, mixing time and dilution condition. We verified that the size of transition vesicles was controlled according to the effective q-value, mixing time, and temperature.
KW - Bicelle
KW - Dilution method
KW - Hydrodynamic focusing
KW - Microfluidic chip
KW - Vesicle
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U2 - 10.1186/s40486-021-00133-4
DO - 10.1186/s40486-021-00133-4
M3 - Letter
AN - SCOPUS:85117783605
SN - 2213-9621
VL - 9
JO - Micro and Nano Systems Letters
JF - Micro and Nano Systems Letters
IS - 1
M1 - 7
ER -