Contractility assessment of human iPSC-derived cardiomyocytes by using a motion vector system and measuring cell impedance

Ayano Satsuka, Sayo Hayashi, Shota Yanagida, Atsushi Ono, Yasunari Kanda

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Predicting drug-induced cardiotoxicity during the non-clinical stage is important to avoid severe consequences in the clinical trials of new drugs. Human iPSC-derived cardiomyocytes (hiPSC-CMs) hold great promise for cardiac safety assessments in drug development. To date, multi-electrode array system (MEA) has been a widely used as a tool for the assessment of proarrhythmic risk with hiPSC-CMs. Recently, new methodologies have been proposed to assess in vitro contractility, such as the force and velocity of cell contraction, using hiPSC-CMs. Herein, we focused on an imaging-based motion vector system (MV) and an electric cell-substrate impedance sensing system (IMP). We compared the output signals of hiPSC-CMs from MV and IMP in detail and observed a clear correlation between the parameters. In addition, we assessed the effects of isoproterenol and verapamil on hiPSC-CM contraction and identified a correlation in the contractile change of parameters obtained with MV and IMP. These results suggest that both assay systems could be used to monitor hiPSC-CM contraction dynamics.

Original languageEnglish
Article number107227
JournalJournal of Pharmacological and Toxicological Methods
Volume118
DOIs
Publication statusPublished - Nov 1 2022

Keywords

  • Cardiomyocyte
  • Contractility
  • Impedance
  • iPSC
  • Motion vector

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology

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