TY - JOUR
T1 - Cryphonectria nitschkei chrysovirus 1 with unique molecular features and a very narrow host range
AU - Shahi, Sabitree
AU - Chiba, Sotaro
AU - Kondo, Hideki
AU - Suzuki, Nobuhiro
N1 - Funding Information:
This study was supported in part by the Ohara Foundation for Agricultural Research , Yomogi Inc., the Joint Usage/Research Center, Institute of Plant Science and Resources, Okayama University to SC, Grants-in-Aid for Scientific Research (A) ( 17H01463 , 15K14663 to NS), (B) (17H03950 to S.C.), and Scientific Research on Innovative Areas from the Ministry of Education, Culture, Sports, Science and Technology, Japan ( 16H06436 , 16H06429 and 16K21723 to N.S. and H.K.). The authors are grateful to Drs. Donald L. Nuss, Bradley I. Hillman, Satoko Kanematsu, Carolina Cornejo, and Daniel Rigling for generous gifts of C. parastica strains EP155, Δdcl2, Δagl2, GH2, and 09G3, and plasmid pXHY9, V. ceratosperma strain AVC53, and C. radicalis strains DR1 and ph1113, and to Drs. Yukiyo Sato and Ida Bagus Andika for fruitful discussion.
Funding Information:
This study was supported in part by the Ohara Foundation for Agricultural Research, Yomogi Inc. the Joint Usage/Research Center, Institute of Plant Science and Resources, Okayama University to SC, Grants-in-Aid for Scientific Research (A) (17H01463, 15K14663 to NS), (B) (17H03950 to S.C.), and Scientific Research on Innovative Areas from the Ministry of Education, Culture, Sports, Science and Technology, Japan (16H06436, 16H06429 and 16K21723 to N.S. and H.K.). The authors are grateful to Drs. Donald L. Nuss, Bradley I. Hillman, Satoko Kanematsu, Carolina Cornejo, and Daniel Rigling for generous gifts of C. parastica strains EP155, ?dcl2, ?agl2, GH2, and 09G3, and plasmid pXHY9, V. ceratosperma strain AVC53, and C. radicalis strains DR1 and ph1113, and to Drs. Yukiyo Sato and Ida Bagus Andika for fruitful discussion.
Publisher Copyright:
© 2020 Elsevier Inc.
PY - 2021/2
Y1 - 2021/2
N2 - Cryphonectria nitschkei chrysovirus 1 (CnCV1), was described earlier from an ascomycetous fungus, Cryphonectria nitschkei strain OB5/11, collected in Japan; its partial sequence was reported a decade ago. Complete sequencing of the four genomic dsRNA segments revealed molecular features similar to but distinct from previously reported members of the family Chrysoviridae. Unique features include the presence of a mini-cistron preceding the major large open reading frame in each genomic segment. Common features include the presence of CAA repeats in the 5′-untranslated regions and conserved terminal sequences. CnCV1-OB5/11 could be laterally transferred to C. nitschkei and its relatives C. radicalis and C. naterciae via coculturing, virion transfection and protoplast fusion, but not to fungal species other than the three species mentioned above, even within the genus Cryphonectria, suggesting a very narrow host range. Phenotypic comparison of a few sets of CnCV1-infected and -free isogenic strains showed symptomless infection in new hosts.
AB - Cryphonectria nitschkei chrysovirus 1 (CnCV1), was described earlier from an ascomycetous fungus, Cryphonectria nitschkei strain OB5/11, collected in Japan; its partial sequence was reported a decade ago. Complete sequencing of the four genomic dsRNA segments revealed molecular features similar to but distinct from previously reported members of the family Chrysoviridae. Unique features include the presence of a mini-cistron preceding the major large open reading frame in each genomic segment. Common features include the presence of CAA repeats in the 5′-untranslated regions and conserved terminal sequences. CnCV1-OB5/11 could be laterally transferred to C. nitschkei and its relatives C. radicalis and C. naterciae via coculturing, virion transfection and protoplast fusion, but not to fungal species other than the three species mentioned above, even within the genus Cryphonectria, suggesting a very narrow host range. Phenotypic comparison of a few sets of CnCV1-infected and -free isogenic strains showed symptomless infection in new hosts.
KW - Chrysovirus
KW - Cryphonectria nitschkei
KW - Cryphonectria parasitica
KW - Cryphonectria radicalis
KW - Fungal virus
KW - Host range
KW - dsRNA
UR - http://www.scopus.com/inward/record.url?scp=85098559306&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85098559306&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2020.11.011
DO - 10.1016/j.virol.2020.11.011
M3 - Article
C2 - 33383414
AN - SCOPUS:85098559306
SN - 0042-6822
VL - 554
SP - 55
EP - 65
JO - Virology
JF - Virology
ER -