TY - JOUR
T1 - Crystalline formate dehydrogenase from Candida methanolica
AU - Izumi, Yoshikazu
AU - Kanzaki, Hiroshi
AU - Morita, Shigeru
AU - Yamada, Hideaki
N1 - Funding Information:
Dr. H. Kanzaki is a recipiento f a JSPS Fellowship for JapaneseJu nior Scientists.
PY - 1987/12/1
Y1 - 1987/12/1
N2 - Formate dehydrogenase (EC 1.2.1.2) was purified about 38-fold with an overall yield of 76% from a methanol-utilizing yeast, Candida methanolica (ATCC26175), in 4 steps and, by adding polyethylene glycol, the enzyme was crystallised for the first time. The final preparation appeared to be homogeneous by the criteria of polyacrylamide electrophoresis and analytical centrifugation. Compared with the yeast formate dehydrogenases so far reported, the purified enzyme exhibited higher specific activity (7.52 U/mg).
AB - Formate dehydrogenase (EC 1.2.1.2) was purified about 38-fold with an overall yield of 76% from a methanol-utilizing yeast, Candida methanolica (ATCC26175), in 4 steps and, by adding polyethylene glycol, the enzyme was crystallised for the first time. The final preparation appeared to be homogeneous by the criteria of polyacrylamide electrophoresis and analytical centrifugation. Compared with the yeast formate dehydrogenases so far reported, the purified enzyme exhibited higher specific activity (7.52 U/mg).
KW - Candida methanolica
KW - Formate dehydrogenase
UR - http://www.scopus.com/inward/record.url?scp=0011174344&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0011174344&partnerID=8YFLogxK
U2 - 10.1111/j.1574-6968.1987.tb02530.x
DO - 10.1111/j.1574-6968.1987.tb02530.x
M3 - Article
AN - SCOPUS:0011174344
SN - 0378-1097
VL - 48
SP - 139
EP - 142
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 1-2
ER -