Cyclic strain induces mouse embryonic stem cell differentiation into vascular smooth muscle cells by activating PDGF receptor β

Nobutaka Shimizu, Kimiko Yamamoto, Syotaro Obi, Shinichiro Kumagaya, Tomomi Masumura, Yasumasa Shimano, Keiji Naruse, Jun K. Yamashita, Takashi Igarashi, Joji Ando

Research output: Contribution to journalArticlepeer-review

101 Citations (Scopus)


Embryonic stem (ES) cells are exposed to fluid-mechanical forces, such as cyclic strain and shear stress, during the process of embryonic development but much remains to be elucidated concerning the role of fluid-mechanical forces in ES cell differentiation. Here, we show that cyclic strain induces vascular smooth muscle cell (VSMC) differentiation in murine ES cells. Flk-1-positive (Flk-1+) ES cells seeded on flexible silicone membranes were subjected to controlled levels of cyclic strain and examined for changes in cell proliferation and expression of various cell lineage markers. When exposed to cyclic strain (4-12% strain, 1 Hz, 24 h), the Flk-1+ ES cells significantly increased in cell number and became oriented perpendicular to the direction of strain. There were dose-dependent increases in the VSMC markers smooth muscle α-actin and smooth muscle-myosin heavy chain at both the protein and gene expression level in response to cyclic strain, whereas expression of the vascular endothelial cell marker Flk-1 decreased, and there were no changes in the other endothelial cell markers (Flt-1, VE-cadherin, and platelet endothelial cell adhesion molecule 1), the blood cell marker CD3, or the epithelial marker keratin. The PDGF receptor β (PDGFRβ) kinase inhibitor AG-1296 completely blocked the cyclic strain-induced increase in cell number and VSMC marker expression. Cyclic strain immediately caused phosphorylation of PDGFRβ in a dose-dependent manner, but neutralizing antibody against PDGF-BB did not block the PDGFRβ phosphorylation. These results suggest that cyclic strain activates PDGFRβ in a ligand-independent manner and that the activation plays a critical role in VSMC differentiation from Flk-1+ ES cells.

Original languageEnglish
Pages (from-to)766-772
Number of pages7
JournalJournal of Applied Physiology
Issue number3
Publication statusPublished - Mar 2008


  • Biomechanics
  • Blood vessel
  • Hemodynamic force

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)


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