Cyclooxygenase 2 expression in otitis media with effusion

Masashi Kakiuchi, Hidetsugu Tsujigiwa, Yorihisa Orita, Hitoshi Nagatsuka, Junko Yoshinobu, Shin Kariya, Shin Ichi Haginomori, Saeko Orita, Kunihiro Fukushima, Mitsuhiro Okano, Noriyuki Nagai, Kazunori Nishizaki

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)


Objectives: To study whether cyclooxygenase 2 (COX-2) plays a role in the development of otitis media with effusion (OME). Study design/methods: An experimental model of endotoxin-induced OME was used in healthy BALB/c mice. Solutions of Salmonella typhimurium endotoxin were injected into the middle ears of the mice, and COX-2 expression in the middle ears was studied using reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization (ISH). Results: Analysis by RT-PCR showed that COX-2 expression in the middle ear increased in a dose-dependent fashion after injection of endotoxin. ISH demonstrated that COX-2 had distinct expression patterns in the epithelium of middle ears with endotoxin-induced OME. Conclusions: COX-2 expression, however, was not detected in normal middle ears. COX-2 expression may be one factor contributing to the development of OME.

Original languageEnglish
Pages (from-to)81-85
Number of pages5
JournalAmerican Journal of Otolaryngology - Head and Neck Medicine and Surgery
Issue number2
Publication statusPublished - Mar 2006

ASJC Scopus subject areas

  • Otorhinolaryngology


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