Cyclopiazonic acid biosynthesis gene cluster gene cpaM is required for speradine A biosynthesis

Masafumi Tokuoka; Tomoki Kikuchi; Yasutomo Shinohara; Akifumi Koyama; Shin Ichiro Iio; Takaaki Kubota; Jun'ichi Kobayashi; Yasuji Koyama; Akira Totsuka; Hitoshi Shindo; Kazuo Sato

doi:10.1080/09168451.2015.1065167

Cyclopiazonic acid biosynthesis gene cluster gene cpaM is required for speradine A biosynthesis

Masafumi Tokuoka, Tomoki Kikuchi, Yasutomo Shinohara, Akifumi Koyama, Shin Ichiro Iio, Takaaki Kubota, Jun'ichi Kobayashi, Yasuji Koyama, Akira Totsuka, Hitoshi Shindo, Kazuo Sato

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Speradine A is a derivative of cyclopiazonic acid (CPA) found in culture of an Aspergillus tamarii isolate. Heterologous expression of a predicted methyltransferase gene, cpaM, in the cpa biosynthesis gene cluster of A. tamarii resulted in the speradine A production in a 2-oxoCPA producing A. oryzae strain, indicating cpaM is involved in the speradine A biosynthesis.

Original language	English
Pages (from-to)	2081-2085
Number of pages	5
Journal	Bioscience, Biotechnology and Biochemistry
Volume	79
Issue number	12
DOIs	https://doi.org/10.1080/09168451.2015.1065167
Publication status	Published - 2015
Externally published	Yes

Keywords

Aspergillus oryzae
Cyclopiazonic acid
Secondary metabolism
Speradine A

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1080/09168451.2015.1065167

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title = "Cyclopiazonic acid biosynthesis gene cluster gene cpaM is required for speradine A biosynthesis",

abstract = "Speradine A is a derivative of cyclopiazonic acid (CPA) found in culture of an Aspergillus tamarii isolate. Heterologous expression of a predicted methyltransferase gene, cpaM, in the cpa biosynthesis gene cluster of A. tamarii resulted in the speradine A production in a 2-oxoCPA producing A. oryzae strain, indicating cpaM is involved in the speradine A biosynthesis.",

keywords = "Aspergillus oryzae, Cyclopiazonic acid, Secondary metabolism, Speradine A",

author = "Masafumi Tokuoka and Tomoki Kikuchi and Yasutomo Shinohara and Akifumi Koyama and Iio, {Shin Ichiro} and Takaaki Kubota and Jun'ichi Kobayashi and Yasuji Koyama and Akira Totsuka and Hitoshi Shindo and Kazuo Sato",

note = "Publisher Copyright: {\textcopyright} 2015 Japan Society for Bioscience, Biotechnology, and Agrochemistry.",

year = "2015",

doi = "10.1080/09168451.2015.1065167",

language = "English",

volume = "79",

pages = "2081--2085",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "0916-8451",

publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

number = "12",

}

TY - JOUR

T1 - Cyclopiazonic acid biosynthesis gene cluster gene cpaM is required for speradine A biosynthesis

AU - Tokuoka, Masafumi

AU - Kikuchi, Tomoki

AU - Shinohara, Yasutomo

AU - Koyama, Akifumi

AU - Iio, Shin Ichiro

AU - Kubota, Takaaki

AU - Kobayashi, Jun'ichi

AU - Koyama, Yasuji

AU - Totsuka, Akira

AU - Shindo, Hitoshi

AU - Sato, Kazuo

PY - 2015

Y1 - 2015

N2 - Speradine A is a derivative of cyclopiazonic acid (CPA) found in culture of an Aspergillus tamarii isolate. Heterologous expression of a predicted methyltransferase gene, cpaM, in the cpa biosynthesis gene cluster of A. tamarii resulted in the speradine A production in a 2-oxoCPA producing A. oryzae strain, indicating cpaM is involved in the speradine A biosynthesis.

AB - Speradine A is a derivative of cyclopiazonic acid (CPA) found in culture of an Aspergillus tamarii isolate. Heterologous expression of a predicted methyltransferase gene, cpaM, in the cpa biosynthesis gene cluster of A. tamarii resulted in the speradine A production in a 2-oxoCPA producing A. oryzae strain, indicating cpaM is involved in the speradine A biosynthesis.

KW - Aspergillus oryzae

KW - Cyclopiazonic acid

KW - Secondary metabolism

KW - Speradine A

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DO - 10.1080/09168451.2015.1065167

M3 - Article

C2 - 26207447

AN - SCOPUS:84946919252

SN - 0916-8451

VL - 79

SP - 2081

EP - 2085

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 12

ER -

Cyclopiazonic acid biosynthesis gene cluster gene cpaM is required for speradine A biosynthesis

Abstract

Keywords

ASJC Scopus subject areas

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