Cytochrome P450 CYP307A1/Spook: A regulator for ecdysone synthesis in insects

Toshiki Namiki, Ryusuke Niwa, Takashi Sakudoh, Ken Ichi Shirai, Hideaki Takeuchi, Hiroshi Kataoka

Research output: Contribution to journalArticlepeer-review

152 Citations (Scopus)


The prothoracic gland (PG) has essential roles in synthesizing and secreting a steroid hormone called ecdysone that is critical for molting and metamorphosis of insects. However, little is known about the genes controlling ecdysteroidogenesis in the PG. To identify genes functioning in the PG of the silkworm, Bombyx mori, we used differential display PCR and focused on a cytochrome P450 gene designated Cyp307a1. Its expression level positively correlates with a change in the hemolymph ecdysteroid titer. In addition, Drosophila Cyp307a1 is encoded in the spook locus, one of the Halloween mutant family members showing a low ecdysone titer in vivo, suggesting that Cyp307a1 is involved in ecdysone synthesis. While Drosophila Cyp307a1 is expressed in the early embryos and adult ovaries, the expression is not observed in the PGs of embryos or third instar larvae. These results suggest a difference in the ecdysone synthesis pathways during larval development in these insects.

Original languageEnglish
Pages (from-to)367-374
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number1
Publication statusPublished - Nov 11 2005
Externally publishedYes


  • Bombyx mori
  • Cyp307a1
  • Cytochrome P450
  • Drosophila melanogaster
  • Ecdysone
  • Halloween mutant
  • Prothoracic gland
  • Spook
  • Steroidogenesis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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