TY - JOUR
T1 - Cytocompatible and antibacterial properties of chitosan-siloxane hybrid spheres
AU - Shirosaki, Yuki
AU - Nakatsukasa, Manato
AU - Yasutomi, Saki
AU - Cruz-Neves, Susana
AU - Hayakawa, Satoshi
AU - Osaka, Akiyoshi
AU - Maeda, Toshinari
AU - Miyazaki, Toshiki
N1 - Funding Information:
This research was funded by Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan (Promotion and Standardization of the Tenure-Track System, "Kojinsenbatsu". We thank Helena McPherson, from Edanz Group (www.edanzediting.com/ac) for editing a drat of this manuscript.
Publisher Copyright:
© 2019 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2019/10/1
Y1 - 2019/10/1
N2 - Microporous spheres in a hybrid system consisting of chitosan and γ-glycidoxypropyltrimethoxysilane (GPTMS) have advantages in a range of applications, e.g., as vehicles for cell transplantation and soft tissue defect filling materials, because of their excellent cytocompatibility with various cells. In this study, microporous chitosan-GPTMS spheres were prepared by dropping chitosan-GPTMS precursor sols,with orwithout a ceriumchloride, into liquid nitrogen using a syringe pump. The droplets were then freeze dried to give the pores of size 10 to 50 μm. The cell culture tests showed that L929 fibroblast-like cells migrated into the micropores larger than 50 μm in diameter, whereasMG63 osteoblast-like cells proliferated well and covered the granule surfaces. The spheres with cerium chloride showed antibacterial properties against both gram-negative and gram-positive bacteria.
AB - Microporous spheres in a hybrid system consisting of chitosan and γ-glycidoxypropyltrimethoxysilane (GPTMS) have advantages in a range of applications, e.g., as vehicles for cell transplantation and soft tissue defect filling materials, because of their excellent cytocompatibility with various cells. In this study, microporous chitosan-GPTMS spheres were prepared by dropping chitosan-GPTMS precursor sols,with orwithout a ceriumchloride, into liquid nitrogen using a syringe pump. The droplets were then freeze dried to give the pores of size 10 to 50 μm. The cell culture tests showed that L929 fibroblast-like cells migrated into the micropores larger than 50 μm in diameter, whereasMG63 osteoblast-like cells proliferated well and covered the granule surfaces. The spheres with cerium chloride showed antibacterial properties against both gram-negative and gram-positive bacteria.
KW - Antibacterial property
KW - Cell behavior
KW - Cerium ion
KW - Chitosan-siloxane hybrid
KW - Microporous spheres
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U2 - 10.3390/polym11101676
DO - 10.3390/polym11101676
M3 - Article
AN - SCOPUS:85073406216
SN - 2073-4360
VL - 11
JO - Polymers
JF - Polymers
IS - 10
M1 - 1676
ER -