Cytotoxic Effect of Methyl Methacrylate on 4 Cultured Fibroblasts

Masayuki Taira, Hiroyuki Nakao, Takuya Matsumoto, Junzo Takahashi

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)


Purpose: The objective of this study was to examine the cytotoxic effect of methyl methacrylate (MMA) monomer on 4 mammalian fibroblasts. Materials and Methods: Four cells - C3H10T1/2, L929, Balb/3T3 clone A31, and MC3T3-E1 - were incubated for 6 days in 24-well microplates filled with 1 mL of Dulbecco's Modified Eagle medium containing MMA monomer that ranged from 0 to 200 mM/L. Cytotoxicity was evaluated by direct cell number count. Results: L929 possessed the largest TC50 value (the amount of MMA that caused 50% cell death) (34 mM/L), and Balb/3T3 clone A31 had the least value (1 mM/L); C3H10T1/2 and MC3T3-E1 had intermediate values (25 mM/L and 16 mM/L, respectively). Conclusion: This study showed that the MMA monomer had dose-dependent cytotoxicity, which varied among the cells tested.

Original languageEnglish
Pages (from-to)311-315
Number of pages5
JournalInternational Journal of Prosthodontics
Issue number4
Publication statusPublished - Jul 2000
Externally publishedYes

ASJC Scopus subject areas

  • Oral Surgery


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