D-Allose as ischemic retina injury inhibitor during rabbit vitrectomy

Masanori Mizote; Kazuyuki Hirooka; Kouki Fukuda; Takehiro Nakamura; Toshifumi Itano; Fumio Shiraga

doi:10.1007/s10384-011-0012-8

D-Allose as ischemic retina injury inhibitor during rabbit vitrectomy

Masanori Mizote, Kazuyuki Hirooka, Kouki Fukuda, Takehiro Nakamura, Toshifumi Itano, Fumio Shiraga

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Purpose: To investigate the protective effects of d-allose, a rare sugar, on pressure-induced ischemia during vitrectomy in the rabbit eye. Methods: The rabbits underwent pars plana vitrectomy, and continuous intraocular irrigation at a perfusion pressure of 140 mmHg was performed for 45 min. Intraocular pressure was regulated by adjusting the height of a bottle of balanced saline solution containing d-allose. Morphometric studies were performed to study the effects of d-allose on the histological changes induced by ischemia in the rabbit retina. Electroretinograms (ERGs) were taken before and 1 and 7 days after vitrectomy. Nitroblue tetrazolium was used as an index of superoxide anion (O ₂ ^•- ) generation. Data were analyzed by use of the unpaired Student's t test. Results: Seven days after ischemia, significant reductions in both number of ganglion cells and the thickness of the inner plexiform layer were observed. d-Allose significantly inhibited ischemic injury of the inner retina (P < 0.05). On postoperative day 7, amplitudes of ERG b-waves were significantly lower in the control group than in the d-allose group (P < 0.05). d-Allose suppressed the production of O₂ ^-. Conclusions: Intraocular irrigation with d-allose during vitrectomy may protect the retina against ischemia-induced damage.

Original language	English
Pages (from-to)	294-300
Number of pages	7
Journal	Japanese Journal of Ophthalmology
Volume	55
Issue number	3
DOIs	https://doi.org/10.1007/s10384-011-0012-8
Publication status	Published - May 2011
Externally published	Yes

Keywords

Neuroprotection
Retinal ischemia
Superoxide anion
Vitrectomy
d-Allose

ASJC Scopus subject areas

Ophthalmology

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10.1007/s10384-011-0012-8

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title = "D-Allose as ischemic retina injury inhibitor during rabbit vitrectomy",

abstract = "Purpose: To investigate the protective effects of d-allose, a rare sugar, on pressure-induced ischemia during vitrectomy in the rabbit eye. Methods: The rabbits underwent pars plana vitrectomy, and continuous intraocular irrigation at a perfusion pressure of 140 mmHg was performed for 45 min. Intraocular pressure was regulated by adjusting the height of a bottle of balanced saline solution containing d-allose. Morphometric studies were performed to study the effects of d-allose on the histological changes induced by ischemia in the rabbit retina. Electroretinograms (ERGs) were taken before and 1 and 7 days after vitrectomy. Nitroblue tetrazolium was used as an index of superoxide anion (O 2 •- ) generation. Data were analyzed by use of the unpaired Student's t test. Results: Seven days after ischemia, significant reductions in both number of ganglion cells and the thickness of the inner plexiform layer were observed. d-Allose significantly inhibited ischemic injury of the inner retina (P < 0.05). On postoperative day 7, amplitudes of ERG b-waves were significantly lower in the control group than in the d-allose group (P < 0.05). d-Allose suppressed the production of O2 -. Conclusions: Intraocular irrigation with d-allose during vitrectomy may protect the retina against ischemia-induced damage.",

keywords = "Neuroprotection, Retinal ischemia, Superoxide anion, Vitrectomy, d-Allose",

author = "Masanori Mizote and Kazuyuki Hirooka and Kouki Fukuda and Takehiro Nakamura and Toshifumi Itano and Fumio Shiraga",

note = "Funding Information: Acknowledgments This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (20592078).",

year = "2011",

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doi = "10.1007/s10384-011-0012-8",

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T1 - D-Allose as ischemic retina injury inhibitor during rabbit vitrectomy

AU - Mizote, Masanori

AU - Hirooka, Kazuyuki

AU - Fukuda, Kouki

AU - Nakamura, Takehiro

AU - Itano, Toshifumi

AU - Shiraga, Fumio

N1 - Funding Information: Acknowledgments This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (20592078).

PY - 2011/5

Y1 - 2011/5

N2 - Purpose: To investigate the protective effects of d-allose, a rare sugar, on pressure-induced ischemia during vitrectomy in the rabbit eye. Methods: The rabbits underwent pars plana vitrectomy, and continuous intraocular irrigation at a perfusion pressure of 140 mmHg was performed for 45 min. Intraocular pressure was regulated by adjusting the height of a bottle of balanced saline solution containing d-allose. Morphometric studies were performed to study the effects of d-allose on the histological changes induced by ischemia in the rabbit retina. Electroretinograms (ERGs) were taken before and 1 and 7 days after vitrectomy. Nitroblue tetrazolium was used as an index of superoxide anion (O 2 •- ) generation. Data were analyzed by use of the unpaired Student's t test. Results: Seven days after ischemia, significant reductions in both number of ganglion cells and the thickness of the inner plexiform layer were observed. d-Allose significantly inhibited ischemic injury of the inner retina (P < 0.05). On postoperative day 7, amplitudes of ERG b-waves were significantly lower in the control group than in the d-allose group (P < 0.05). d-Allose suppressed the production of O2 -. Conclusions: Intraocular irrigation with d-allose during vitrectomy may protect the retina against ischemia-induced damage.

AB - Purpose: To investigate the protective effects of d-allose, a rare sugar, on pressure-induced ischemia during vitrectomy in the rabbit eye. Methods: The rabbits underwent pars plana vitrectomy, and continuous intraocular irrigation at a perfusion pressure of 140 mmHg was performed for 45 min. Intraocular pressure was regulated by adjusting the height of a bottle of balanced saline solution containing d-allose. Morphometric studies were performed to study the effects of d-allose on the histological changes induced by ischemia in the rabbit retina. Electroretinograms (ERGs) were taken before and 1 and 7 days after vitrectomy. Nitroblue tetrazolium was used as an index of superoxide anion (O 2 •- ) generation. Data were analyzed by use of the unpaired Student's t test. Results: Seven days after ischemia, significant reductions in both number of ganglion cells and the thickness of the inner plexiform layer were observed. d-Allose significantly inhibited ischemic injury of the inner retina (P < 0.05). On postoperative day 7, amplitudes of ERG b-waves were significantly lower in the control group than in the d-allose group (P < 0.05). d-Allose suppressed the production of O2 -. Conclusions: Intraocular irrigation with d-allose during vitrectomy may protect the retina against ischemia-induced damage.

KW - Neuroprotection

KW - Retinal ischemia

KW - Superoxide anion

KW - Vitrectomy

KW - d-Allose

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D-Allose as ischemic retina injury inhibitor during rabbit vitrectomy

Abstract

Keywords

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