DDX3 DEAD-box RNA helicase is required for hepatitis C virus RNA replication

Yasuo Ariumi; Misao Kuroki; Ken Ichi Abe; Hiromichi Dansako; Masanori Ikeda; Takaji Wakita; Nobuyuki Kato

doi:10.1128/JVI.01517-07

DDX3 DEAD-box RNA helicase is required for hepatitis C virus RNA replication

Yasuo Ariumi, Misao Kuroki, Ken Ichi Abe, Hiromichi Dansako, Masanori Ikeda, Takaji Wakita, Nobuyuki Kato

Research output: Contribution to journal › Article › peer-review

210 Citations (Scopus)

Abstract

DDX3, a DEAD-box RNA helicase, binds to the hepatitis C virus (HCV) core protein. However, the role(s) of DDX3 in HCV replication is still not understood. Here we demonstrate that the accumulation of both genome-length HCV RNA (HCV-O, genotype 1b) and its replicon RNA were significantly suppressed in HuH-7-derived cells expressing short hairpin RNA targeted to DDX3 by lentivirus vector transduction. As well, RNA replication of JFH1 (genotype 2a) and release of the core into the culture supernatants were suppressed in DDX3 knockdown cells after inoculation of the cell culture-generated HCVcc. Thus, DDX3 is required for HCV RNA replication.

Original language	English
Pages (from-to)	13922-13926
Number of pages	5
Journal	Journal of Virology
Volume	81
Issue number	24
DOIs	https://doi.org/10.1128/JVI.01517-07
Publication status	Published - Dec 2007

ASJC Scopus subject areas

Microbiology
Immunology
Insect Science
Virology

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/JVI.01517-07

Cite this

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title = "DDX3 DEAD-box RNA helicase is required for hepatitis C virus RNA replication",

abstract = "DDX3, a DEAD-box RNA helicase, binds to the hepatitis C virus (HCV) core protein. However, the role(s) of DDX3 in HCV replication is still not understood. Here we demonstrate that the accumulation of both genome-length HCV RNA (HCV-O, genotype 1b) and its replicon RNA were significantly suppressed in HuH-7-derived cells expressing short hairpin RNA targeted to DDX3 by lentivirus vector transduction. As well, RNA replication of JFH1 (genotype 2a) and release of the core into the culture supernatants were suppressed in DDX3 knockdown cells after inoculation of the cell culture-generated HCVcc. Thus, DDX3 is required for HCV RNA replication.",

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AU - Ariumi, Yasuo

AU - Kuroki, Misao

AU - Abe, Ken Ichi

AU - Dansako, Hiromichi

AU - Ikeda, Masanori

AU - Wakita, Takaji

AU - Kato, Nobuyuki

PY - 2007/12

Y1 - 2007/12

N2 - DDX3, a DEAD-box RNA helicase, binds to the hepatitis C virus (HCV) core protein. However, the role(s) of DDX3 in HCV replication is still not understood. Here we demonstrate that the accumulation of both genome-length HCV RNA (HCV-O, genotype 1b) and its replicon RNA were significantly suppressed in HuH-7-derived cells expressing short hairpin RNA targeted to DDX3 by lentivirus vector transduction. As well, RNA replication of JFH1 (genotype 2a) and release of the core into the culture supernatants were suppressed in DDX3 knockdown cells after inoculation of the cell culture-generated HCVcc. Thus, DDX3 is required for HCV RNA replication.

AB - DDX3, a DEAD-box RNA helicase, binds to the hepatitis C virus (HCV) core protein. However, the role(s) of DDX3 in HCV replication is still not understood. Here we demonstrate that the accumulation of both genome-length HCV RNA (HCV-O, genotype 1b) and its replicon RNA were significantly suppressed in HuH-7-derived cells expressing short hairpin RNA targeted to DDX3 by lentivirus vector transduction. As well, RNA replication of JFH1 (genotype 2a) and release of the core into the culture supernatants were suppressed in DDX3 knockdown cells after inoculation of the cell culture-generated HCVcc. Thus, DDX3 is required for HCV RNA replication.

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DDX3 DEAD-box RNA helicase is required for hepatitis C virus RNA replication

Abstract

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