Delivery of dsRNA with lactic acid bacteria for RNA interference.

A. Kuwahara; Masashi Arita; Masahiko Sisido; Takashi Ohtsuki

Delivery of dsRNA with lactic acid bacteria for RNA interference.

A. Kuwahara, Masashi Arita, Masahiko Sisido, Takashi Ohtsuki

Research output: Contribution to journal › Article › peer-review

Abstract

RNA interference (RNAi) mechanism is promising for gene-targeted therapy. In this study, Lactobacillus casei were engineered to produce long double-stranded RNA (dsRNA) or short hairpin RNA (shRNA) for RNAi-delivery to the intestine. We prepared two kinds of L. casei; one produces shRNA which works in mammalian cells, and the other produces long dsRNA which works in nematode. We confirmed the expression of shRNA and dsRNA in the transformed L. casei by Northern blotting and RT-PCR analyses.

Original language	English
Pages (from-to)	413-414
Number of pages	2
Journal	Nucleic acids symposium series (2004)
Issue number	51
Publication status	Published - 2007

ASJC Scopus subject areas

Medicine(all)

Cite this

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abstract = "RNA interference (RNAi) mechanism is promising for gene-targeted therapy. In this study, Lactobacillus casei were engineered to produce long double-stranded RNA (dsRNA) or short hairpin RNA (shRNA) for RNAi-delivery to the intestine. We prepared two kinds of L. casei; one produces shRNA which works in mammalian cells, and the other produces long dsRNA which works in nematode. We confirmed the expression of shRNA and dsRNA in the transformed L. casei by Northern blotting and RT-PCR analyses.",

author = "A. Kuwahara and Masashi Arita and Masahiko Sisido and Takashi Ohtsuki",

year = "2007",

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AU - Arita, Masashi

AU - Sisido, Masahiko

AU - Ohtsuki, Takashi

PY - 2007

Y1 - 2007

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AB - RNA interference (RNAi) mechanism is promising for gene-targeted therapy. In this study, Lactobacillus casei were engineered to produce long double-stranded RNA (dsRNA) or short hairpin RNA (shRNA) for RNAi-delivery to the intestine. We prepared two kinds of L. casei; one produces shRNA which works in mammalian cells, and the other produces long dsRNA which works in nematode. We confirmed the expression of shRNA and dsRNA in the transformed L. casei by Northern blotting and RT-PCR analyses.

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JF - Nucleic acids symposium series (2004)

IS - 51

ER -

Delivery of dsRNA with lactic acid bacteria for RNA interference.

Abstract

ASJC Scopus subject areas

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