TY - JOUR
T1 - Demonstration of receptors specific for connective tissue growth factor on a human chondrocytic cell line (HCS-2/8)
AU - Nishida, Takashi
AU - Nakanishi, Tohru
AU - Shimo, Tsuyoshi
AU - Asano, Masahiro
AU - Hattori, Takako
AU - Tamatani, Takuya
AU - Tezuka, Katsunari
AU - Takigawa, Masaharu
N1 - Funding Information:
We thank Dr. Kojiro Takahashi for useful discussions, Dr. Takuo Fujisawa for technical assistance, and Mrs. Etsuko Fujisawa for secretarial assistance. This work was supported in part by Grants-in-Aid for Scienti®c Research from the Ministry of Education, Science, Sports, and Culture of Japan and grants from the Sagawa Cancer Research Foundation, the Naito Foundation, and the Nakatomi Health Science Foundation.
PY - 1998/6/29
Y1 - 1998/6/29
N2 - The presence of receptors specific for connective tissue growth factor (CTGF) was demonstrated on a human chondrosarcoma-derived chondrocytic cell line, HCS-2/8. The binding of 125I-labeled recombinant CTGF to HCS-2/8 cells was inhibited by unlabeled CTGF but not by PDGF-BB or bFGF. Scatchard analysis revealed the presence of two classes of binding sites with Kd values of 18.6 and 259 nM on cells. A cross-linking study revealed the formation of 125I-CTGF-receptor complex with an apparent molecular weight of 280 kDa. The 125I-CTGF-receptor complex disappeared almost completely on the addition of unlabeled CTGF but not PDGF-BB or bFGF. In addition, the 125I-CTGF-receptor complex was immunoprecipitated with anti-CTGF antiserum but not with anti-PDGF receptor antiserum. These findings suggest that CTGF directly binds to specific receptor molecules on HCS-2/8 cells.
AB - The presence of receptors specific for connective tissue growth factor (CTGF) was demonstrated on a human chondrosarcoma-derived chondrocytic cell line, HCS-2/8. The binding of 125I-labeled recombinant CTGF to HCS-2/8 cells was inhibited by unlabeled CTGF but not by PDGF-BB or bFGF. Scatchard analysis revealed the presence of two classes of binding sites with Kd values of 18.6 and 259 nM on cells. A cross-linking study revealed the formation of 125I-CTGF-receptor complex with an apparent molecular weight of 280 kDa. The 125I-CTGF-receptor complex disappeared almost completely on the addition of unlabeled CTGF but not PDGF-BB or bFGF. In addition, the 125I-CTGF-receptor complex was immunoprecipitated with anti-CTGF antiserum but not with anti-PDGF receptor antiserum. These findings suggest that CTGF directly binds to specific receptor molecules on HCS-2/8 cells.
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U2 - 10.1006/bbrc.1998.8895
DO - 10.1006/bbrc.1998.8895
M3 - Article
C2 - 9647791
AN - SCOPUS:0032577910
SN - 0006-291X
VL - 247
SP - 905
EP - 909
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -