TY - JOUR
T1 - Dentin Phosphophoryn Promotes Cellular Migration of Human Dental Pulp Cells
AU - Yasuda, Yoshiyuki
AU - Izumikawa, Masanobu
AU - Okamoto, Kuniaki
AU - Tsukuba, Takayuki
AU - Saito, Takashi
N1 - Funding Information:
This work was supported by Grant-in-Aid for Scientific Research (Nos. 18659536 and 18791407) from the Japan Society for the Promotion of Science and by a Grant from the Research Center, Health Sciences University of Hokkaido.
PY - 2008/5
Y1 - 2008/5
N2 - Dentin phosphophoryn (DPP) is a dentin sialophosphoprotein gene product that has an RGD motif and repeat sequences of aspartic acid and phosphoserine. To date, the function of DPP in the early stage of reparative dentin formation still remains unclear. The objective of this study was to evaluate the effects of DPP on pulp cell migration and proliferation. DPP promoted cell migration in a concentration-dependent manner, thus increasing it by about 3-fold at 1000 ng/mL compared with the control, but it had no effect on cell proliferation. Dephosphorylated DPP also promoted cell migration, similarly to DPP. However, cell migration was significantly suppressed by the addition of αvβ3 integrin antibody to the medium. Furthermore, porcine DPP-derived RGD peptide, but not its mutant RAD peptide, significantly promoted cell migration. These results indicated that the RGD motif of DPP plays an important role in the migration of human dental pulp cells.
AB - Dentin phosphophoryn (DPP) is a dentin sialophosphoprotein gene product that has an RGD motif and repeat sequences of aspartic acid and phosphoserine. To date, the function of DPP in the early stage of reparative dentin formation still remains unclear. The objective of this study was to evaluate the effects of DPP on pulp cell migration and proliferation. DPP promoted cell migration in a concentration-dependent manner, thus increasing it by about 3-fold at 1000 ng/mL compared with the control, but it had no effect on cell proliferation. Dephosphorylated DPP also promoted cell migration, similarly to DPP. However, cell migration was significantly suppressed by the addition of αvβ3 integrin antibody to the medium. Furthermore, porcine DPP-derived RGD peptide, but not its mutant RAD peptide, significantly promoted cell migration. These results indicated that the RGD motif of DPP plays an important role in the migration of human dental pulp cells.
KW - Dental pulp cells
KW - RGD motif
KW - dentin sialophosphoprotein
KW - migration
KW - phosphophoryn
KW - reparative dentin
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U2 - 10.1016/j.joen.2008.02.018
DO - 10.1016/j.joen.2008.02.018
M3 - Article
C2 - 18436037
AN - SCOPUS:43049133680
SN - 0099-2399
VL - 34
SP - 575
EP - 578
JO - Journal of Endodontics
JF - Journal of Endodontics
IS - 5
ER -