Detection of 3-nitrotyrosine in atmospheric environments via a highperformance liquid chromatography-electrochemical detector system

Tatsuo Ito; Keiki Ogino; Kenjiro Nagaoka; Kei Takemoto; Rina Nishiyama; Yurika Shimizu

doi:10.3791/58371

Detection of 3-nitrotyrosine in atmospheric environments via a highperformance liquid chromatography-electrochemical detector system

Tatsuo Ito, Keiki Ogino, Kenjiro Nagaoka, Kei Takemoto, Rina Nishiyama, Yurika Shimizu

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

3-nitrotyrosine (3-NT) is generated from the tyrosine residue in atmospheric bio-aerosol proteins via a reaction with ozone (O ₃ ) and nitrogen dioxide (NO ₂ ). Stable 3-NT is a specific marker for oxidative damage and is reported to have a promotive effect to elicit allergies. In the present study, we report the development of a highly sensitive assay to quantify 3-NT in air sampler filters to collect < 2.5 µm of particulate matter (PM _2.5 ) from urban environmental air, including bio-aerosol. In this method, a 6 mm-diameter round hole was cut from the filters of air samplers and mixed with a nonspecific protease cocktail in order to hydrolyze proteins. Protein samples digested to the amino acid level were tested for 3-NT using a high-performance liquid chromatography-electrochemical detector (HPLC-ECD). The maximum 3-NT content was detected in a prefilter for PM of sizes from 4.5 to 7.3 µm, with a detection limit of 1.13 pg/m ³ .

Original language	English
Article number	e58371
Journal	Journal of Visualized Experiments
Volume	2019
Issue number	143
DOIs	https://doi.org/10.3791/58371
Publication status	Published - Jan 2019

Keywords

3-nitrotyrosine
Air atmospheric environmental pollution
Environmental sciences
HPLC-ECD
Issue 143
Nitrogen dioxide
Ozone
Particulate matter
Protein modification

ASJC Scopus subject areas

Neuroscience(all)
Chemical Engineering(all)
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3791/58371

Cite this

@article{56c25b40bc024a04a5b1ef074b6ede07,

title = "Detection of 3-nitrotyrosine in atmospheric environments via a highperformance liquid chromatography-electrochemical detector system",

abstract = " 3-nitrotyrosine (3-NT) is generated from the tyrosine residue in atmospheric bio-aerosol proteins via a reaction with ozone (O 3 ) and nitrogen dioxide (NO 2 ). Stable 3-NT is a specific marker for oxidative damage and is reported to have a promotive effect to elicit allergies. In the present study, we report the development of a highly sensitive assay to quantify 3-NT in air sampler filters to collect < 2.5 µm of particulate matter (PM 2.5 ) from urban environmental air, including bio-aerosol. In this method, a 6 mm-diameter round hole was cut from the filters of air samplers and mixed with a nonspecific protease cocktail in order to hydrolyze proteins. Protein samples digested to the amino acid level were tested for 3-NT using a high-performance liquid chromatography-electrochemical detector (HPLC-ECD). The maximum 3-NT content was detected in a prefilter for PM of sizes from 4.5 to 7.3 µm, with a detection limit of 1.13 pg/m 3 . ",

keywords = "3-nitrotyrosine, Air atmospheric environmental pollution, Environmental sciences, HPLC-ECD, Issue 143, Nitrogen dioxide, Ozone, Particulate matter, Protein modification",

author = "Tatsuo Ito and Keiki Ogino and Kenjiro Nagaoka and Kei Takemoto and Rina Nishiyama and Yurika Shimizu",

note = "Funding Information: The authors thank Masayuki Kubo of the US Environmental Protection Agency for his help with the TSP/PM2.5 sampling. This work was supported in part by JSPS KAKENHI Grant Number JP16K15373 and JP18H03039. Publisher Copyright: {\textcopyright} 2019 Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.",

year = "2019",

month = jan,

doi = "10.3791/58371",

language = "English",

volume = "2019",

journal = "Journal of Visualized Experiments",

issn = "1940-087X",

publisher = "MYJoVE Corporation",

number = "143",

}

TY - JOUR

T1 - Detection of 3-nitrotyrosine in atmospheric environments via a highperformance liquid chromatography-electrochemical detector system

AU - Ito, Tatsuo

AU - Ogino, Keiki

AU - Nagaoka, Kenjiro

AU - Takemoto, Kei

AU - Nishiyama, Rina

AU - Shimizu, Yurika

N1 - Funding Information: The authors thank Masayuki Kubo of the US Environmental Protection Agency for his help with the TSP/PM2.5 sampling. This work was supported in part by JSPS KAKENHI Grant Number JP16K15373 and JP18H03039. Publisher Copyright: © 2019 Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.

PY - 2019/1

Y1 - 2019/1

N2 - 3-nitrotyrosine (3-NT) is generated from the tyrosine residue in atmospheric bio-aerosol proteins via a reaction with ozone (O 3 ) and nitrogen dioxide (NO 2 ). Stable 3-NT is a specific marker for oxidative damage and is reported to have a promotive effect to elicit allergies. In the present study, we report the development of a highly sensitive assay to quantify 3-NT in air sampler filters to collect < 2.5 µm of particulate matter (PM 2.5 ) from urban environmental air, including bio-aerosol. In this method, a 6 mm-diameter round hole was cut from the filters of air samplers and mixed with a nonspecific protease cocktail in order to hydrolyze proteins. Protein samples digested to the amino acid level were tested for 3-NT using a high-performance liquid chromatography-electrochemical detector (HPLC-ECD). The maximum 3-NT content was detected in a prefilter for PM of sizes from 4.5 to 7.3 µm, with a detection limit of 1.13 pg/m 3 .

AB - 3-nitrotyrosine (3-NT) is generated from the tyrosine residue in atmospheric bio-aerosol proteins via a reaction with ozone (O 3 ) and nitrogen dioxide (NO 2 ). Stable 3-NT is a specific marker for oxidative damage and is reported to have a promotive effect to elicit allergies. In the present study, we report the development of a highly sensitive assay to quantify 3-NT in air sampler filters to collect < 2.5 µm of particulate matter (PM 2.5 ) from urban environmental air, including bio-aerosol. In this method, a 6 mm-diameter round hole was cut from the filters of air samplers and mixed with a nonspecific protease cocktail in order to hydrolyze proteins. Protein samples digested to the amino acid level were tested for 3-NT using a high-performance liquid chromatography-electrochemical detector (HPLC-ECD). The maximum 3-NT content was detected in a prefilter for PM of sizes from 4.5 to 7.3 µm, with a detection limit of 1.13 pg/m 3 .

KW - 3-nitrotyrosine

KW - Air atmospheric environmental pollution

KW - Environmental sciences

KW - HPLC-ECD

KW - Issue 143

KW - Nitrogen dioxide

KW - Ozone

KW - Particulate matter

KW - Protein modification

UR - http://www.scopus.com/inward/record.url?scp=85062074226&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85062074226&partnerID=8YFLogxK

U2 - 10.3791/58371

DO - 10.3791/58371

M3 - Article

C2 - 30774123

AN - SCOPUS:85062074226

SN - 1940-087X

VL - 2019

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

IS - 143

M1 - e58371

ER -

Detection of 3-nitrotyrosine in atmospheric environments via a highperformance liquid chromatography-electrochemical detector system

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this