Detection of anti-ebolavirus antibodies in Ghanaian pigs

Hirohito Ogawa; Kenji Ohya; Raphael Ayizanga; Hiroko Miyamoto; Asako Shigeno; Masao Yamada; Yasuhiro Takashima; Miho Inoue-Murayama; Ayato Takada; Boniface Baboreka Kayang

doi:10.1292/jvms.22-0186

Detection of anti-ebolavirus antibodies in Ghanaian pigs

Hirohito Ogawa, Kenji Ohya, Raphael Ayizanga, Hiroko Miyamoto, Asako Shigeno, Masao Yamada, Yasuhiro Takashima, Miho Inoue-Murayama, Ayato Takada, Boniface Baboreka Kayang

Research output: Contribution to journal › Article › peer-review

Abstract

Some filoviruses such as ebolaviruses and marburgviruses, cause hemorrhagic fever in humans and nonhuman primates. Pigs are suggested to play a potential role in the filovirus ecology. We investigated the seroprevalence of filovirus infection in pigs in Ghana. Using a viral glycoprotein (GP)-based enzyme-linked immunosorbent assay, we detected filovirus-specific immunoglobulin G antibodies in 5 of 139 samples. These positive sera showed specificities to four different filovirus species. Particularly, two of the positive sera reacted to GPs of two African ebolaviruses (i.e., Ebola virus and Taï Forest virus) in Western blotting. Our results suggest that these Ghanaian pigs were exposed to multiple filoviruses and emphasize the importance of continuous monitoring of filovirus infection in pig populations in West African countries.

Original language	English
Journal	The Journal of veterinary medical science
DOIs	https://doi.org/10.1292/jvms.22-0186
Publication status	E-pub ahead of print - Sept 20 2022
Externally published	Yes

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title = "Detection of anti-ebolavirus antibodies in Ghanaian pigs",

abstract = "Some filoviruses such as ebolaviruses and marburgviruses, cause hemorrhagic fever in humans and nonhuman primates. Pigs are suggested to play a potential role in the filovirus ecology. We investigated the seroprevalence of filovirus infection in pigs in Ghana. Using a viral glycoprotein (GP)-based enzyme-linked immunosorbent assay, we detected filovirus-specific immunoglobulin G antibodies in 5 of 139 samples. These positive sera showed specificities to four different filovirus species. Particularly, two of the positive sera reacted to GPs of two African ebolaviruses (i.e., Ebola virus and Ta{\"i} Forest virus) in Western blotting. Our results suggest that these Ghanaian pigs were exposed to multiple filoviruses and emphasize the importance of continuous monitoring of filovirus infection in pig populations in West African countries.",

author = "Hirohito Ogawa and Kenji Ohya and Raphael Ayizanga and Hiroko Miyamoto and Asako Shigeno and Masao Yamada and Yasuhiro Takashima and Miho Inoue-Murayama and Ayato Takada and {Baboreka Kayang}, Boniface",

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month = sep,

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doi = "10.1292/jvms.22-0186",

language = "English",

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TY - JOUR

T1 - Detection of anti-ebolavirus antibodies in Ghanaian pigs

AU - Ogawa, Hirohito

AU - Ohya, Kenji

AU - Ayizanga, Raphael

AU - Miyamoto, Hiroko

AU - Shigeno, Asako

AU - Yamada, Masao

AU - Takashima, Yasuhiro

AU - Inoue-Murayama, Miho

AU - Takada, Ayato

AU - Baboreka Kayang, Boniface

PY - 2022/9/20

Y1 - 2022/9/20

N2 - Some filoviruses such as ebolaviruses and marburgviruses, cause hemorrhagic fever in humans and nonhuman primates. Pigs are suggested to play a potential role in the filovirus ecology. We investigated the seroprevalence of filovirus infection in pigs in Ghana. Using a viral glycoprotein (GP)-based enzyme-linked immunosorbent assay, we detected filovirus-specific immunoglobulin G antibodies in 5 of 139 samples. These positive sera showed specificities to four different filovirus species. Particularly, two of the positive sera reacted to GPs of two African ebolaviruses (i.e., Ebola virus and Taï Forest virus) in Western blotting. Our results suggest that these Ghanaian pigs were exposed to multiple filoviruses and emphasize the importance of continuous monitoring of filovirus infection in pig populations in West African countries.

AB - Some filoviruses such as ebolaviruses and marburgviruses, cause hemorrhagic fever in humans and nonhuman primates. Pigs are suggested to play a potential role in the filovirus ecology. We investigated the seroprevalence of filovirus infection in pigs in Ghana. Using a viral glycoprotein (GP)-based enzyme-linked immunosorbent assay, we detected filovirus-specific immunoglobulin G antibodies in 5 of 139 samples. These positive sera showed specificities to four different filovirus species. Particularly, two of the positive sera reacted to GPs of two African ebolaviruses (i.e., Ebola virus and Taï Forest virus) in Western blotting. Our results suggest that these Ghanaian pigs were exposed to multiple filoviruses and emphasize the importance of continuous monitoring of filovirus infection in pig populations in West African countries.

U2 - 10.1292/jvms.22-0186

DO - 10.1292/jvms.22-0186

M3 - Article

C2 - 36123040

SN - 0916-7250

JO - Journal of Veterinary Medical Science

JF - Journal of Veterinary Medical Science

ER -

Detection of anti-ebolavirus antibodies in Ghanaian pigs

Abstract

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