Detection of Epstein-Barr Virus RNA and Related Antigens in Non-Neoplastic Lymphoid Lesions

Yuji Tonoyama, Norihiro Teramoto, Ashit Baran Sarker, Tadashi Yoshino, Kazuhiko Hayashi, Kiyoshi Takahashi, Tadaatsu Akagi

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7 Citations (Scopus)


To elucidate the latent state and reactivation of Epstein-Barr virus (EBV) in non-neoplastic lymphoid lesions, we investigated 144 non-neoplastic lymphoid lesions by in situ hybridization (ISH) to detect the expression of EBV-encoded small RNAs (EBER)-1 and BCRF-1 and by immunostaining for latent membrane protein (LMP)-1 and ZEBRA. ISH for EBER-1 detected EBER-1-positive cells (EPC) in 31 of the 144 examined lesions (22%). EPC were detected in 4 of 49 cases of nonspecific lymphoid hyperplasia, in 16 of 20 abscess-forming granulomatous lymphadenitis (AFGL), 5 of 25 Kikuchi's disease, and in 3 of 3 infectious mononucleosis. LMP-1 was expressed in 6 of 124 non-neoplastic lymphoid lesions (4.8%). LMP-1-positive cells were observed in 6 of the 31 EBER-1-positive cases (19%). EPC were detected significantly more frequently in LMP-1- and ZEBRA-positive specimens than in the LMP-1- and ZEBRA-negative specimens. BCRF-1 was expressed in 4 of 11 cases examined: 2 of 3 AFGL, 1 of 2 Kikuchi's disease, and in the 1 case of atypical lymphoid hyperplasia. This study suggests that Epstein-Barr virus is prevalent and can be reactivated in the lymph nodes effaced by destructive inflammation, such as AFGL. Such inflammation may provide a local milieu that is conducive for EBV to enter the lytic cycle.

Original languageEnglish
Pages (from-to)89-96
Number of pages8
JournalActa medica Okayama
Issue number2
Publication statusPublished - Apr 1996


  • BCRF-I
  • EBER-I
  • LMP-I
  • Lymphoid lesion

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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