Detection of Fibrillization Process of Amyloid Beta Protein Using Arrayed Biosensor with Liposome Encapsulating Fluorescent Molecules

R. Imamura, T. Shimanouchi, N. Murata, K. Yamashita, M. Fukuzawa, M. Noda

    Research output: Contribution to journalConference articlepeer-review

    2 Citations (Scopus)

    Abstract

    We have successfully detected fibrillization process of amyloid-beta(1-40) protein (Aβ(1-40)) as causative compounds of Alzheimer's disease by our developed bio array sensing system utilizing different phospholipid liposomes encapsulating fluorescent molecules. The fluorescent intensity is dependent on the leakage of fluorescent molecules through perturbed lipid membrane of liposome. Therefore, the intensity considerably depends on the liposome-Aβ interaction strength on fibrillization process of Aβ(1-40). It is noted that the behavior of the fluorescent intensity corresponded to that of our cantilever sensor when measuring the interaction. Moreover, difference in Aβ(1-40) concentration or liposome-Aβ(1-40) interaction strength was successfully discriminated by principal component analysis. Consequently, we believe that the fluorescent liposome sensor is very effective for discriminating aggregation and fibrillization processes and concentrations of Aβ(1-40).

    Original languageEnglish
    Pages (from-to)1414-1417
    Number of pages4
    JournalProcedia Engineering
    Volume168
    DOIs
    Publication statusPublished - 2016
    Event30th Eurosensors Conference, Eurosensors 2016 - Budapest, Hungary
    Duration: Sept 4 2016Sept 7 2016

    Keywords

    • Amyloid Beta
    • Array
    • Biosensor
    • Fluorescence
    • Liposome
    • Pricipal Component Analysis

    ASJC Scopus subject areas

    • General Engineering

    Fingerprint

    Dive into the research topics of 'Detection of Fibrillization Process of Amyloid Beta Protein Using Arrayed Biosensor with Liposome Encapsulating Fluorescent Molecules'. Together they form a unique fingerprint.

    Cite this