Detection of hepatitis C virus RNA in circulating immune complexes by RT-PCR

Takechiyo Morita, Hajime Hada, Norio Koide, Hidenori Shiraha, Toshiyuki Shinji, Masaki Nakamura, Kouzo Ujike, Masaki Wato, Hiroyuki Shimomura, Takao Tsuji

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Materials and Methods: We examined HCV-RNA immunoprecipitated with anti-human IgG, A and M antibodies by reverse transcription-polymerase chain reaction. Results: In thirty-nine (91%) of 43 patients, composed of 35 chronic hepatitis (CH) and 8 liver cirrhosis (LC), HCV-RNA was detected in the CIC. All 43 patients analyzed were classified into the following three categories; HCV-RNA was detected only in the supernatant (S pattern, 4 patients), both in the supernatant and the precipitate (SP pattern, 27 patients), and only in the precipitate (P pattern, 12 patients). SP pattern was most common in chronic HCV infection, and the frequency of SP pattern decreased with the progression of liver disease. P pattern was significantly more frequent in patients with higher γ-globulin, levels, histologically indicated LC, and antibody to HCV envelope protein. Conclusion: We found that HCV formed a CIC in most patients with chronic HCV infection, and that the formation of CIC might be related to the stage of chronic HCV infection.

Original languageEnglish
Pages (from-to)582-585
Number of pages4
Issue number9
Publication statusPublished - Jul 4 1996


  • Circulating immune complex
  • Hepatitis C virus
  • RNA
  • RT-PCR

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology


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