Detection of the silkworm-pathogenic virus genomes by PCR.

Seiji Kageyasu; Tohoru Hayakawa; Haruhiko Isawa; Shinichiro Asano; Ken Sahara; Toshihiko Iizuka; Hisanori Bando

doi:10.11416/kontyushigen1930.66.477

Detection of the silkworm-pathogenic virus genomes by PCR.

Seiji Kageyasu, Tohoru Hayakawa, Haruhiko Isawa, Shinichiro Asano, Ken Sahara, Toshihiko Iizuka, Hisanori Bando

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The five viruses, Bombyx mori densonucleosis virus type 1 (BmDNV-1), - type 2 (BmDNV-2), Bombyx mori nuclear polyhedrosis virus (BmNPV), Bombyx mori cytoplasmic polyhedrosis virus (BmCPV), and Infectious flacherie virus (IFV), are the agents causing fetal disease of the silkworm. The aim of this study is to establish a simple and highly sensitive PCR system for detection of all the viruses. A combination of the RT-PCR and the nested PCR was found to be effective to amplify DNA and/or RNA virus genome sequences in a reaction maintaining high sensitivity and specificity. In this report, the primer design and the condition of the PCR for the detection of all the viruses are described.

Original language	English
Pages (from-to)	477-483
Number of pages	7
Journal	Journal of Sericultural Science of Japan
Volume	66
Issue number	6
DOIs	https://doi.org/10.11416/kontyushigen1930.66.477
Publication status	Published - Jan 1 1997
Externally published	Yes

Keywords

BmCPV
BmDNV
BmNPV
IFV
RT-PCR
nested PCR

ASJC Scopus subject areas

Polymers and Plastics

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10.11416/kontyushigen1930.66.477

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title = "Detection of the silkworm-pathogenic virus genomes by PCR.",

abstract = "The five viruses, Bombyx mori densonucleosis virus type 1 (BmDNV-1), - type 2 (BmDNV-2), Bombyx mori nuclear polyhedrosis virus (BmNPV), Bombyx mori cytoplasmic polyhedrosis virus (BmCPV), and Infectious flacherie virus (IFV), are the agents causing fetal disease of the silkworm. The aim of this study is to establish a simple and highly sensitive PCR system for detection of all the viruses. A combination of the RT-PCR and the nested PCR was found to be effective to amplify DNA and/or RNA virus genome sequences in a reaction maintaining high sensitivity and specificity. In this report, the primer design and the condition of the PCR for the detection of all the viruses are described.",

keywords = "BmCPV, BmDNV, BmNPV, IFV, RT-PCR, nested PCR",

author = "Seiji Kageyasu and Tohoru Hayakawa and Haruhiko Isawa and Shinichiro Asano and Ken Sahara and Toshihiko Iizuka and Hisanori Bando",

year = "1997",

month = jan,

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doi = "10.11416/kontyushigen1930.66.477",

language = "English",

volume = "66",

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journal = "Journal of Sericultural Science of Japan",

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AU - Kageyasu, Seiji

AU - Hayakawa, Tohoru

AU - Isawa, Haruhiko

AU - Asano, Shinichiro

AU - Sahara, Ken

AU - Iizuka, Toshihiko

AU - Bando, Hisanori

PY - 1997/1/1

Y1 - 1997/1/1

N2 - The five viruses, Bombyx mori densonucleosis virus type 1 (BmDNV-1), - type 2 (BmDNV-2), Bombyx mori nuclear polyhedrosis virus (BmNPV), Bombyx mori cytoplasmic polyhedrosis virus (BmCPV), and Infectious flacherie virus (IFV), are the agents causing fetal disease of the silkworm. The aim of this study is to establish a simple and highly sensitive PCR system for detection of all the viruses. A combination of the RT-PCR and the nested PCR was found to be effective to amplify DNA and/or RNA virus genome sequences in a reaction maintaining high sensitivity and specificity. In this report, the primer design and the condition of the PCR for the detection of all the viruses are described.

AB - The five viruses, Bombyx mori densonucleosis virus type 1 (BmDNV-1), - type 2 (BmDNV-2), Bombyx mori nuclear polyhedrosis virus (BmNPV), Bombyx mori cytoplasmic polyhedrosis virus (BmCPV), and Infectious flacherie virus (IFV), are the agents causing fetal disease of the silkworm. The aim of this study is to establish a simple and highly sensitive PCR system for detection of all the viruses. A combination of the RT-PCR and the nested PCR was found to be effective to amplify DNA and/or RNA virus genome sequences in a reaction maintaining high sensitivity and specificity. In this report, the primer design and the condition of the PCR for the detection of all the viruses are described.

KW - BmCPV

KW - BmDNV

KW - BmNPV

KW - IFV

KW - RT-PCR

KW - nested PCR

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DO - 10.11416/kontyushigen1930.66.477

M3 - Article

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JO - Journal of Sericultural Science of Japan

JF - Journal of Sericultural Science of Japan

IS - 6

ER -

Detection of the silkworm-pathogenic virus genomes by PCR.

Abstract

Keywords

ASJC Scopus subject areas

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