@inbook{2e4061b719b34537866f1b147eda2d8a,
title = "Detection of thermospermine and spermine by HPLC in plants",
abstract = "Thermospermine, a structural isomer of spermine, is widely spread in the plant kingdom and has recently been shown to play a key role in the repression of xylem differentiation in vascular plants. However, a standard high-performance liquid chromatography (HPLC) protocol for detecting polyamines as their dansyl derivative cannot distinguish themospermine from spermine. These isomers become separated from each other after benzoylation. In this chapter, we describe a simple protocol for extraction, benzoylation, and HPLC detection of thermospermine and spermine with other polyamines from plant material.",
keywords = "Arabidopsis, Benzoylation, HPLC, Spermine, Thermospermine",
author = "Taku Takahashi and Ayaka Takano and Kakehi, {Jun Ichi}",
year = "2018",
doi = "10.1007/978-1-4939-7398-9_6",
language = "English",
volume = "1694",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "69--73",
booktitle = "Methods in Molecular Biology",
}