TY - JOUR
T1 - Detection techniques for epitranscriptomic marks
AU - Ofusa, Ken
AU - Chijimatsu, Ryota
AU - Ishii, Hideshi
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology Grants 20H00541 and 21K19526 (to H.I.). Partial support was received from Princess Takamatsu Cancer Research Fund, Senshin Medical Research Foundation, and Mitsubishi Foundation (to H.I.).
Publisher Copyright:
© 2022 the American Physiological Society.
PY - 2022/4
Y1 - 2022/4
N2 - Similar to epigenetic DNA modification, RNA can be methylated and altered for stability and processing. RNA modifications, namely, epitranscriptomes, involve the following three functions: writing, erasing, and reading of marks. Methods for measurement and position detection are useful for the assessment of cellular function and human disease biomarkers. After pyrimidine 5-methylcytosine was reported for the first time a hundred years ago, numerous techniques have been developed for studying nucleotide modifications, including RNAs. Recent studies have focused on high-throughput and direct measurements for investigating the precise function of epitranscriptomes, including the characterization of severe acute respiratory syndrome coronavirus 2. The current study presents an overview of the development of detection techniques for epitranscriptomic marks and briefs about the recent progress in this field.
AB - Similar to epigenetic DNA modification, RNA can be methylated and altered for stability and processing. RNA modifications, namely, epitranscriptomes, involve the following three functions: writing, erasing, and reading of marks. Methods for measurement and position detection are useful for the assessment of cellular function and human disease biomarkers. After pyrimidine 5-methylcytosine was reported for the first time a hundred years ago, numerous techniques have been developed for studying nucleotide modifications, including RNAs. Recent studies have focused on high-throughput and direct measurements for investigating the precise function of epitranscriptomes, including the characterization of severe acute respiratory syndrome coronavirus 2. The current study presents an overview of the development of detection techniques for epitranscriptomic marks and briefs about the recent progress in this field.
KW - epitranscriptomics
KW - methylation
KW - RNA
KW - techniques
KW - virus
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U2 - 10.1152/ajpcell.00460.2021
DO - 10.1152/ajpcell.00460.2021
M3 - Review article
C2 - 35294846
AN - SCOPUS:85128245350
SN - 0363-6143
VL - 322
SP - C787-C793
JO - American Journal of Physiology
JF - American Journal of Physiology
IS - 4
ER -
