TY - JOUR
T1 - Determination of cell fate in skeletal muscle following BMP gene transfer by in vivo electroporation
AU - Kawai, Mariko
AU - Ohmori, Yu Ki
AU - Nishino, Mai
AU - Yoshida, Masayo
AU - Tabata, Kaori
AU - Hirota, Do Saku
AU - Ryu-Mon, Ayako
AU - Yamamoto, Hiromitsu
AU - Sonobe, Junya
AU - Kataoka, Yo Hei
AU - Shiotsu, Noriko
AU - Ikegame, Mika
AU - Maruyama, Hiroki
AU - Yamamoto, Toshio
AU - Bessho, Kazuhisa
AU - Ohura, Kiyoshi
N1 - Publisher Copyright:
© M. Kawai et al., 2017.
PY - 2017/5/5
Y1 - 2017/5/5
N2 - We previously developed a novel method for gene transfer, which combined a non-viral gene expression vector with transcutaneous in vivo electroporation. We applied this method to transfer the bone morphogenetic protein (BMP) gene and induce ectopic bone formation in rat skeletal muscles. At present, it remains unclear which types of cells can differentiate into osteogenic cells after BMP gene transfer by in vivo electroporation. Two types of stem cells in skeletal muscle can differentiate into osteogenic cells: muscle-derived stem cells, and bone marrow-derived stem cells in the blood. In the present study, we transferred the BMP gene into rat skeletal muscles. We then stained tissues for several muscle-derived stem cell markers (e.g., Pax7, M-cadherin), muscle regenerationrelated markers (e.g., Myod1, myogenin), and an inflammatory cell marker (CD68) to follow cell differentiation over time. Our results indicate that, in the absence of BMP, the cell population undergoes muscle regeneration, whereas in its presence, it can differentiate into osteogenic cells. Commitment towards either muscle regeneration or induction of ectopic bone formation appears to occur five to seven days after BMP gene transfer.
AB - We previously developed a novel method for gene transfer, which combined a non-viral gene expression vector with transcutaneous in vivo electroporation. We applied this method to transfer the bone morphogenetic protein (BMP) gene and induce ectopic bone formation in rat skeletal muscles. At present, it remains unclear which types of cells can differentiate into osteogenic cells after BMP gene transfer by in vivo electroporation. Two types of stem cells in skeletal muscle can differentiate into osteogenic cells: muscle-derived stem cells, and bone marrow-derived stem cells in the blood. In the present study, we transferred the BMP gene into rat skeletal muscles. We then stained tissues for several muscle-derived stem cell markers (e.g., Pax7, M-cadherin), muscle regenerationrelated markers (e.g., Myod1, myogenin), and an inflammatory cell marker (CD68) to follow cell differentiation over time. Our results indicate that, in the absence of BMP, the cell population undergoes muscle regeneration, whereas in its presence, it can differentiate into osteogenic cells. Commitment towards either muscle regeneration or induction of ectopic bone formation appears to occur five to seven days after BMP gene transfer.
KW - BMP
KW - Cell fate
KW - Gene transfer
KW - In vivo electroporation
KW - Skeletal muscle
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U2 - 10.4081/ejh.2017.2772
DO - 10.4081/ejh.2017.2772
M3 - Article
C2 - 28735515
AN - SCOPUS:85020035099
SN - 1121-760X
VL - 61
SP - 65
EP - 70
JO - Basic and Applied Histochemistry
JF - Basic and Applied Histochemistry
IS - 2
M1 - 2772
ER -