TY - JOUR
T1 - Determination of histone by membrane filter collection with 3,4,5,6-tetrafluoro-2-carboxyphenylfluorone and manganese (II)
AU - Tominaga, Hiroshi
AU - Kadobayashi, Hiroko
AU - Yamaguchi, Takako
AU - Kamino, Shinichiro
AU - Nishimura, Michiko
AU - Fujita, Yoshikazu
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2006
Y1 - 2006
N2 - We reported on a spectrophotometric determination of DNA binding protein, histone, with 3,4,5,6-tetrafluoro-2-carboxyphenylfluorone and manganese(II) in a previous paper. In the present work, preconcentration method for the determination of histone, which involves the filtration of a histone - 3,4,5,6-tetrafluoro-2-carboxyphenylfluorone - manganese (II) aggregate on a membrane filter, followed by its redissolution or tristimulus colorimetry, is described. Each of these methods is about 10-times more sensitive than the usual spectrophotometry.
AB - We reported on a spectrophotometric determination of DNA binding protein, histone, with 3,4,5,6-tetrafluoro-2-carboxyphenylfluorone and manganese(II) in a previous paper. In the present work, preconcentration method for the determination of histone, which involves the filtration of a histone - 3,4,5,6-tetrafluoro-2-carboxyphenylfluorone - manganese (II) aggregate on a membrane filter, followed by its redissolution or tristimulus colorimetry, is described. Each of these methods is about 10-times more sensitive than the usual spectrophotometry.
KW - 3,4,5,6-tetrafluoro-2-carboxyphenylfluorone
KW - Histone determination
KW - Manganese(II)
KW - Membrane filter
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U2 - 10.2116/bunsekikagaku.55.127
DO - 10.2116/bunsekikagaku.55.127
M3 - Article
AN - SCOPUS:33646177941
SN - 0525-1931
VL - 55
SP - 127
EP - 131
JO - BUNSEKI KAGAKU
JF - BUNSEKI KAGAKU
IS - 2
ER -