Determination of oxidized low-density lipoproteins (ox-LDL) versus ox-LDL/β2GPI complexes for the assessment of autoimmune-mediated atherosclerosis

Luis R. Lopez; Tammy R. Buckner; Beth L. Hurley; Kazuko Kobayashi; Eiji Matsuura

doi:10.1196/annals.1398.036

Determination of oxidized low-density lipoproteins (ox-LDL) versus ox-LDL/β2GPI complexes for the assessment of autoimmune-mediated atherosclerosis

Luis R. Lopez, Tammy R. Buckner, Beth L. Hurley, Kazuko Kobayashi, Eiji Matsuura

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

22 Citations (Scopus)

Abstract

The immunolocalization of oxidized low-density lipoproteins (ox-LDL), β2-glycoprotein I (β₂GPI), CD4⁺/CD8⁺ immunoreactive lymphocytes, and immunoglobulins in atherosclerotic lesions strongly suggested an active participation of the immune system in atherogenesis. Oxidative stress leading to ox-LDL production is thought to play a central role in both the initiation and progression of atherosclerosis. ox-LDL is highly proinflammatory and chemotactic for macrophage/monocyte and immune cells. Enzyme-linked immunosorbent assays (ELISAs) to measure circulating ox-LDL have been developed and are being currently used to assess oxidative stress as risk factor or marker of atherosclerotic disease. ox-LDL interacts with β₂GPI and circulating ox-LDL/β₂GPI complexes have been demonstrated in patients with systemic lupus erythematosus (SLE) and antiphospholipid syndrome (APS). It has been postulated that β₂GPI binds ox-LDL to neutralize its proinflammatory and proatherosclerotic effects. Because β₂GPI is ubiquitous in plasma, its interaction with ox-LDL may mask oxidized epitopes recognized by capture antibodies potentially interfering with immunoassays results. The measurement of ox-LDL/β₂GPI complexes may circumvent this interference representing a more physiological and accurate way of measuring ox-LDL.

Original language	English
Title of host publication	Autoimmunity, Part A Basic Principles and New Diagnostic Tools
Publisher	Blackwell Publishing Inc.
Pages	303-310
Number of pages	8
ISBN (Print)	1573316636, 9781573316637
DOIs	https://doi.org/10.1196/annals.1398.036
Publication status	Published - Aug 2007

Publication series

Name	Annals of the New York Academy of Sciences
Volume	1109
ISSN (Print)	0077-8923
ISSN (Electronic)	1749-6632

Keywords

Atherosclerosis
Autoimmunity
ELISA
Oxidative stress
Oxidized low-density lipoprotein (ox-LDL)
β2-glycoprotein I (βGPI)

ASJC Scopus subject areas

Neuroscience(all)
Biochemistry, Genetics and Molecular Biology(all)
History and Philosophy of Science

Access to Document

10.1196/annals.1398.036

Cite this

Lopez, L. R., Buckner, T. R., Hurley, B. L., Kobayashi, K., & Matsuura, E. (2007). Determination of oxidized low-density lipoproteins (ox-LDL) versus ox-LDL/β2GPI complexes for the assessment of autoimmune-mediated atherosclerosis. In Autoimmunity, Part A Basic Principles and New Diagnostic Tools (pp. 303-310). (Annals of the New York Academy of Sciences; Vol. 1109). Blackwell Publishing Inc.. https://doi.org/10.1196/annals.1398.036

Determination of oxidized low-density lipoproteins (ox-LDL) versus ox-LDL/β2GPI complexes for the assessment of autoimmune-mediated atherosclerosis. / Lopez, Luis R.; Buckner, Tammy R.; Hurley, Beth L. et al.
Autoimmunity, Part A Basic Principles and New Diagnostic Tools. Blackwell Publishing Inc., 2007. p. 303-310 (Annals of the New York Academy of Sciences; Vol. 1109).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Lopez, LR, Buckner, TR, Hurley, BL, Kobayashi, K & Matsuura, E 2007, Determination of oxidized low-density lipoproteins (ox-LDL) versus ox-LDL/β2GPI complexes for the assessment of autoimmune-mediated atherosclerosis. in Autoimmunity, Part A Basic Principles and New Diagnostic Tools. Annals of the New York Academy of Sciences, vol. 1109, Blackwell Publishing Inc., pp. 303-310. https://doi.org/10.1196/annals.1398.036

Lopez LR, Buckner TR, Hurley BL, Kobayashi K, Matsuura E. Determination of oxidized low-density lipoproteins (ox-LDL) versus ox-LDL/β2GPI complexes for the assessment of autoimmune-mediated atherosclerosis. In Autoimmunity, Part A Basic Principles and New Diagnostic Tools. Blackwell Publishing Inc. 2007. p. 303-310. (Annals of the New York Academy of Sciences). doi: 10.1196/annals.1398.036

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abstract = "The immunolocalization of oxidized low-density lipoproteins (ox-LDL), β2-glycoprotein I (β2GPI), CD4+/CD8+ immunoreactive lymphocytes, and immunoglobulins in atherosclerotic lesions strongly suggested an active participation of the immune system in atherogenesis. Oxidative stress leading to ox-LDL production is thought to play a central role in both the initiation and progression of atherosclerosis. ox-LDL is highly proinflammatory and chemotactic for macrophage/monocyte and immune cells. Enzyme-linked immunosorbent assays (ELISAs) to measure circulating ox-LDL have been developed and are being currently used to assess oxidative stress as risk factor or marker of atherosclerotic disease. ox-LDL interacts with β2GPI and circulating ox-LDL/β2GPI complexes have been demonstrated in patients with systemic lupus erythematosus (SLE) and antiphospholipid syndrome (APS). It has been postulated that β2GPI binds ox-LDL to neutralize its proinflammatory and proatherosclerotic effects. Because β2GPI is ubiquitous in plasma, its interaction with ox-LDL may mask oxidized epitopes recognized by capture antibodies potentially interfering with immunoassays results. The measurement of ox-LDL/β2GPI complexes may circumvent this interference representing a more physiological and accurate way of measuring ox-LDL.",

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AB - The immunolocalization of oxidized low-density lipoproteins (ox-LDL), β2-glycoprotein I (β2GPI), CD4+/CD8+ immunoreactive lymphocytes, and immunoglobulins in atherosclerotic lesions strongly suggested an active participation of the immune system in atherogenesis. Oxidative stress leading to ox-LDL production is thought to play a central role in both the initiation and progression of atherosclerosis. ox-LDL is highly proinflammatory and chemotactic for macrophage/monocyte and immune cells. Enzyme-linked immunosorbent assays (ELISAs) to measure circulating ox-LDL have been developed and are being currently used to assess oxidative stress as risk factor or marker of atherosclerotic disease. ox-LDL interacts with β2GPI and circulating ox-LDL/β2GPI complexes have been demonstrated in patients with systemic lupus erythematosus (SLE) and antiphospholipid syndrome (APS). It has been postulated that β2GPI binds ox-LDL to neutralize its proinflammatory and proatherosclerotic effects. Because β2GPI is ubiquitous in plasma, its interaction with ox-LDL may mask oxidized epitopes recognized by capture antibodies potentially interfering with immunoassays results. The measurement of ox-LDL/β2GPI complexes may circumvent this interference representing a more physiological and accurate way of measuring ox-LDL.

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Determination of oxidized low-density lipoproteins (ox-LDL) versus ox-LDL/β2GPI complexes for the assessment of autoimmune-mediated atherosclerosis

Abstract

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Keywords

ASJC Scopus subject areas

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