Determination of urinary protein with o-sulfophenylfluorone-niobium(V) complex

Yurina Masui; Haruna Sakai; Yurie Kawakami; Shinichiro Kamino; Takako Yamaguchi; Yoshikazu Fujita

doi:10.2116/bunsekikagaku.57.637

Determination of urinary protein with o-sulfophenylfluorone-niobium(V) complex

Yurina Masui, Haruna Sakai, Yurie Kawakami, Shinichiro Kamino, Takako Yamaguchi, Yoshikazu Fujita

Graduate School of Medicine Dentistry and Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

A spectrophotometric method was established for the determination of urinary protein with an o-sulfophenylfluorone-niobium(V) {SPF-Nb(V)} complex. This method is about 1.2-times more sensitive than the pyrogallol red-molybdenum(VI) method (PR method), and can determine trace amount of urinary protein. There was good for agreement between the results obtained for urinary protein by this method and those obtained by PR method (r = 0.914). The binding process between the {SPF-Nb(V)} complex and HSA was studied by determining the thermodynamic parameters. Moreover, upon application of this method to a protein assay kit, a mixed reagent was constructed and a simple sensitive method was developed for a visual detection of protein. The proposed method should be useful for a simple, rapid and sensitive determinations of urinary protein.

Original language	English
Pages (from-to)	637-642
Number of pages	6
Journal	BUNSEKI KAGAKU
Volume	57
Issue number	8
DOIs	https://doi.org/10.2116/bunsekikagaku.57.637
Publication status	Published - 2008

Keywords

Spectrophotometry
Urinary protein
Visual detection of protein
o-sulfophenylfluorone-niobium(V) complex

ASJC Scopus subject areas

Analytical Chemistry

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10.2116/bunsekikagaku.57.637

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title = "Determination of urinary protein with o-sulfophenylfluorone-niobium(V) complex",

abstract = "A spectrophotometric method was established for the determination of urinary protein with an o-sulfophenylfluorone-niobium(V) {SPF-Nb(V)} complex. This method is about 1.2-times more sensitive than the pyrogallol red-molybdenum(VI) method (PR method), and can determine trace amount of urinary protein. There was good for agreement between the results obtained for urinary protein by this method and those obtained by PR method (r = 0.914). The binding process between the {SPF-Nb(V)} complex and HSA was studied by determining the thermodynamic parameters. Moreover, upon application of this method to a protein assay kit, a mixed reagent was constructed and a simple sensitive method was developed for a visual detection of protein. The proposed method should be useful for a simple, rapid and sensitive determinations of urinary protein.",

keywords = "Spectrophotometry, Urinary protein, Visual detection of protein, o-sulfophenylfluorone-niobium(V) complex",

author = "Yurina Masui and Haruna Sakai and Yurie Kawakami and Shinichiro Kamino and Takako Yamaguchi and Yoshikazu Fujita",

year = "2008",

doi = "10.2116/bunsekikagaku.57.637",

language = "English",

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pages = "637--642",

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publisher = "Japan Society for Analytical Chemistry",

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TY - JOUR

T1 - Determination of urinary protein with o-sulfophenylfluorone-niobium(V) complex

AU - Masui, Yurina

AU - Sakai, Haruna

AU - Kawakami, Yurie

AU - Kamino, Shinichiro

AU - Yamaguchi, Takako

AU - Fujita, Yoshikazu

PY - 2008

Y1 - 2008

N2 - A spectrophotometric method was established for the determination of urinary protein with an o-sulfophenylfluorone-niobium(V) {SPF-Nb(V)} complex. This method is about 1.2-times more sensitive than the pyrogallol red-molybdenum(VI) method (PR method), and can determine trace amount of urinary protein. There was good for agreement between the results obtained for urinary protein by this method and those obtained by PR method (r = 0.914). The binding process between the {SPF-Nb(V)} complex and HSA was studied by determining the thermodynamic parameters. Moreover, upon application of this method to a protein assay kit, a mixed reagent was constructed and a simple sensitive method was developed for a visual detection of protein. The proposed method should be useful for a simple, rapid and sensitive determinations of urinary protein.

AB - A spectrophotometric method was established for the determination of urinary protein with an o-sulfophenylfluorone-niobium(V) {SPF-Nb(V)} complex. This method is about 1.2-times more sensitive than the pyrogallol red-molybdenum(VI) method (PR method), and can determine trace amount of urinary protein. There was good for agreement between the results obtained for urinary protein by this method and those obtained by PR method (r = 0.914). The binding process between the {SPF-Nb(V)} complex and HSA was studied by determining the thermodynamic parameters. Moreover, upon application of this method to a protein assay kit, a mixed reagent was constructed and a simple sensitive method was developed for a visual detection of protein. The proposed method should be useful for a simple, rapid and sensitive determinations of urinary protein.

KW - Spectrophotometry

KW - Urinary protein

KW - Visual detection of protein

KW - o-sulfophenylfluorone-niobium(V) complex

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ER -

Determination of urinary protein with o-sulfophenylfluorone-niobium(V) complex

Abstract

Keywords

ASJC Scopus subject areas

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