Determination of urinary protein with o-sulfophenylfluorone-niobium(V) complex

Yurina Masui, Haruna Sakai, Yurie Kawakami, Shinichiro Kamino, Takako Yamaguchi, Yoshikazu Fujita

Research output: Contribution to journalArticlepeer-review


A spectrophotometric method was established for the determination of urinary protein with an o-sulfophenylfluorone-niobium(V) {SPF-Nb(V)} complex. This method is about 1.2-times more sensitive than the pyrogallol red-molybdenum(VI) method (PR method), and can determine trace amount of urinary protein. There was good for agreement between the results obtained for urinary protein by this method and those obtained by PR method (r = 0.914). The binding process between the {SPF-Nb(V)} complex and HSA was studied by determining the thermodynamic parameters. Moreover, upon application of this method to a protein assay kit, a mixed reagent was constructed and a simple sensitive method was developed for a visual detection of protein. The proposed method should be useful for a simple, rapid and sensitive determinations of urinary protein.

Original languageEnglish
Pages (from-to)637-642
Number of pages6
Issue number8
Publication statusPublished - 2008


  • Spectrophotometry
  • Urinary protein
  • Visual detection of protein
  • o-sulfophenylfluorone-niobium(V) complex

ASJC Scopus subject areas

  • Analytical Chemistry


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