Development and characterization of microsatellite loci in the endangered Tricyrtis ishiiana (Convallariaceae), a local endemic plant in Japan

Hiroaki Setoguchi; Yuki Mitsui; Hajime Ikeda; Naofumi Nomura; Atsushi Tamura

doi:10.1007/s10592-008-9620-3

Development and characterization of microsatellite loci in the endangered Tricyrtis ishiiana (Convallariaceae), a local endemic plant in Japan

Hiroaki Setoguchi, Yuki Mitsui, Hajime Ikeda, Naofumi Nomura, Atsushi Tamura

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Nine microsatellite loci were isolated and characterized for the endangered and tertiary relict perennial herb, Tricyrtis ishiiana. The number of alleles ranged from 2 to 33. The expected (H _E) and observed (H _O) heterozygosities were 0.207-0.944 and 0.215-0.813, respectively, from 96 individuals on one population. Five loci exhibited significantly fewer heterozygotes than expected under Hardy-Weinberg equilibrium (P < 0.05). These primers amplifying microsatellites in this species may provide a useful tool for population genetics to establish conservation strategy.

Original language	English
Pages (from-to)	705-707
Number of pages	3
Journal	Conservation Genetics
Volume	10
Issue number	3
DOIs	https://doi.org/10.1007/s10592-008-9620-3
Publication status	Published - Jun 2009
Externally published	Yes

Keywords

Conservation genetics
Convallariaceae
Heterozygosity
Microsatellite
Tricyrtis ishiiana

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Genetics

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10.1007/s10592-008-9620-3

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title = "Development and characterization of microsatellite loci in the endangered Tricyrtis ishiiana (Convallariaceae), a local endemic plant in Japan",

abstract = "Nine microsatellite loci were isolated and characterized for the endangered and tertiary relict perennial herb, Tricyrtis ishiiana. The number of alleles ranged from 2 to 33. The expected (H E) and observed (H O) heterozygosities were 0.207-0.944 and 0.215-0.813, respectively, from 96 individuals on one population. Five loci exhibited significantly fewer heterozygotes than expected under Hardy-Weinberg equilibrium (P < 0.05). These primers amplifying microsatellites in this species may provide a useful tool for population genetics to establish conservation strategy.",

keywords = "Conservation genetics, Convallariaceae, Heterozygosity, Microsatellite, Tricyrtis ishiiana",

author = "Hiroaki Setoguchi and Yuki Mitsui and Hajime Ikeda and Naofumi Nomura and Atsushi Tamura",

note = "Funding Information: Acknowledgements We are grateful to Ms Akiko Sakai (Kanagawa Prefecture Natural Environment Conservation Center) and Asuka Noda (Kyoto University) for their cooperation in collection materials or extracting DNA, respectively. This study was supported by grant for Conservation Genetics for Endangered Plants from Kanagawa Prefecture Natural Environment Conservation Center. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.",

year = "2009",

month = jun,

doi = "10.1007/s10592-008-9620-3",

language = "English",

volume = "10",

pages = "705--707",

journal = "Conservation Genetics",

issn = "1566-0621",

publisher = "Springer Netherlands",

number = "3",

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T1 - Development and characterization of microsatellite loci in the endangered Tricyrtis ishiiana (Convallariaceae), a local endemic plant in Japan

AU - Setoguchi, Hiroaki

AU - Mitsui, Yuki

AU - Ikeda, Hajime

AU - Nomura, Naofumi

AU - Tamura, Atsushi

N1 - Funding Information: Acknowledgements We are grateful to Ms Akiko Sakai (Kanagawa Prefecture Natural Environment Conservation Center) and Asuka Noda (Kyoto University) for their cooperation in collection materials or extracting DNA, respectively. This study was supported by grant for Conservation Genetics for Endangered Plants from Kanagawa Prefecture Natural Environment Conservation Center. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.

PY - 2009/6

Y1 - 2009/6

N2 - Nine microsatellite loci were isolated and characterized for the endangered and tertiary relict perennial herb, Tricyrtis ishiiana. The number of alleles ranged from 2 to 33. The expected (H E) and observed (H O) heterozygosities were 0.207-0.944 and 0.215-0.813, respectively, from 96 individuals on one population. Five loci exhibited significantly fewer heterozygotes than expected under Hardy-Weinberg equilibrium (P < 0.05). These primers amplifying microsatellites in this species may provide a useful tool for population genetics to establish conservation strategy.

AB - Nine microsatellite loci were isolated and characterized for the endangered and tertiary relict perennial herb, Tricyrtis ishiiana. The number of alleles ranged from 2 to 33. The expected (H E) and observed (H O) heterozygosities were 0.207-0.944 and 0.215-0.813, respectively, from 96 individuals on one population. Five loci exhibited significantly fewer heterozygotes than expected under Hardy-Weinberg equilibrium (P < 0.05). These primers amplifying microsatellites in this species may provide a useful tool for population genetics to establish conservation strategy.

KW - Conservation genetics

KW - Convallariaceae

KW - Heterozygosity

KW - Microsatellite

KW - Tricyrtis ishiiana

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DO - 10.1007/s10592-008-9620-3

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SN - 1566-0621

VL - 10

SP - 705

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JO - Conservation Genetics

JF - Conservation Genetics

IS - 3

ER -

Development and characterization of microsatellite loci in the endangered Tricyrtis ishiiana (Convallariaceae), a local endemic plant in Japan

Abstract

Keywords

ASJC Scopus subject areas

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