Development of a real-time loop-mediated isothermal amplification assay for the sensitive and rapid detection of Listeria monocytogenes

L. Ye; Y. Li; J. Zhao; Z. Zhang; H. Meng; H. Yan; S. I. Miyoshi; L. Shi

doi:10.1111/lam.12429

Development of a real-time loop-mediated isothermal amplification assay for the sensitive and rapid detection of Listeria monocytogenes

L. Ye, Y. Li, J. Zhao, Z. Zhang, H. Meng, H. Yan, S. I. Miyoshi, L. Shi

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

A real-time loop-mediated isothermal amplification (RealAmp) assay for the detection of Listeria was developed. The RealAmp assay, using primers specific for the hemolysin-encoding hlyA gene, was verified using Listeria monocytogenes strains (n = 58) from different regions of the world. Both the sensitivity and specificity of the RealAmp assay were high. The RealAmp assay could detect 10³ CFU ml^-1 within 30 min. A comparative evaluation of the RealAmp assay, the API Listeria assay, and the real-time PCR assay revealed that the RealAmp assay is simpler, faster, and has a higher specificity than the other two assays.

Original language	English
Pages (from-to)	85-90
Number of pages	6
Journal	Letters in Applied Microbiology
Volume	61
Issue number	1
DOIs	https://doi.org/10.1111/lam.12429
Publication status	Published - Jul 1 2015

Keywords

API Listeria
ESEQuant tube scanner
Listeria monocytogenes
Real-time PCR
RealAmp

ASJC Scopus subject areas

Applied Microbiology and Biotechnology

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10.1111/lam.12429

Cite this

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AU - Ye, L.

AU - Li, Y.

AU - Zhao, J.

AU - Zhang, Z.

AU - Meng, H.

AU - Yan, H.

AU - Miyoshi, S. I.

AU - Shi, L.

PY - 2015/7/1

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Development of a real-time loop-mediated isothermal amplification assay for the sensitive and rapid detection of Listeria monocytogenes

Abstract

Keywords

ASJC Scopus subject areas

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